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bioelisa READ HIGHLIGHTED CHANGES bioelisa HAV IgM 3000-1098 96 tests Procedural flow chart / Esquema del procedimiento / Schematischer Ablauf / Schéma de la procédure / Schema del procedimento / Esquema do procedimento Dilute Samples ( 1/200), DO NOT dilute Controls Diluir Muestras (1/200), NO diluir los Controles Proben verdünnen (1/200), DIE Kontrollen nicht verdünnen Diluer Échantillons (1/200), NE PAS diluer les contrôles Diluire Campioni (1/200), NON diluire i Controlli Diluir Amostras (1/200), NÃO diluir os Controles Pipette Samples Diluent: 100 µl/well Dispensar Diluyente de las Muestras: 100 µl/pocillo Probenverdünnungsmittel verteilen: 100 µl/Vertiefung Dispenser Diluant des Échantillons: 100 µl/puits Dispensare Diluente dei Campioni: 100 µl/pozzetto Pipetar Diluente de Amostras: 100 µl/pocinho Pipette pre-diluted Samples: 5 µl/well Dispensar Muestras prediluidas: 5 µl/pocillo Vorverdünnte Proben verteilen: 5 µl/Vertiefung Dispenser Échantillons pré dilués: 5 µl/puits Dispensare Campioni prediluiti: 5 µl/pozzetto Pipetar Amostras pré-diluídas: 5 µl/pocinho Pipette Controls: 100 µl/well Dispensar Controles: 100 µl/pocillo Kontrollen verteilen: 100 µl/Vertiefung Dispenser Contrôles: 100 µl/puits Dispensare Controlli: 100 µl/pozzetto Pipetar Controles: 100 µl/pocinho Incubate 1 hour at 37°C Incubar 1 hora a 37°C 1 Stunde bei 37°C inkubieren Incuber 1 heure à 37°C Incubare 1 ora a 37°C Incubar 1 hora a 37°C Wash 6x / Lavar 6x Waschen 6x / Laver 6x Lavare 6x / Lavar 6x Pipette Conjugate and Virus: 50 µl/well and 50 µl/well Dispensar el Conjugado y el Virus: 50 µl/pocillo y 50 µl/pocillo Konjugat und Virus pipettieren: 50 µl/Vertiefung und 50 µl/Vertiefung Dispenser le Conjugué et le Virus: 50 µl/puits et 50 µl/puits Dispensare il Coniugato e il Virus: 50 µl/pozzetto e 50 µl/pozzetto Pipetar o Conjugado e o Vírus: 50 µl/pocinho e 50 µl/pocinho Incubate 1 hour at 37°C Incubar 1 hora a 37°C 1 Stunde bei 37°C inkubieren Incuber 1 heure à 37°C Incubare 1 ora a 37°C Incubar 1 hora a 37°C Wash 6x / Lavar 6x Waschen 6x / Laver 6x Lavare 6x / Lavar 6x Pipette Substrate: 100 µl/well Dispensar el Sustrato: 100 µl/pocillo Substrat pipettieren: 100 µl/Vertiefung Dispenser le Substrat: 100 µl/puits Dispensare il Substrato: 100 µl/pozzetto Pipetar o Substrato: 100 µl/pocinho Incubate 30 minutes at RT Incubar 30 minutos a TA 30 Minuten bei RT inkubieren Incuber 30 minutes à TA Incubare 30 minuti a TA Incubar 30 minutos a TA Pipette Stopping Solution: 100 µl/well Dispensar la Solución de Parada: 100 µl/pocillo Stopplösung zufügen: 100 µl/Vertiefung Dispenser la Solution d’Arrêt: 100 µl/puits Dispensare la Soluzione Bloccante: 100 µl/pozzetto Pipetar a Solução de Bloqueio: 100 µl/pocinho Cut-off: NCx + (PCx/4) Valor umbral: CNx + (CPx/4) Schwellenwert: NKx + (PKx/4) Valeur seuil: CNx + (CPx/4) Valore di soglia: CNx + (CPx/4) Cut-off: CNx + (CPx/4) Read at 450 nm Leer a 450 nm Bei 450 nm ablesen Lire à 450 nm Leggere a 450 nm Ler a 450 nm BIOKIT, S.A. - Can Malé s/n - 08186 Lliçà d’Amunt - Barcelona - SPAIN 3000-1098 R07 06.2012 procedural flow chart.doc
