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Title: Simultaneous detection of eight viruses and two viroids affecting stone fruit trees by using a unique polyprobe. Journal: European Journal of Plant Pathology Authors: Ana Peiró, Vicente Pallás and Jesús Ángel Sánchez-Navarro* Instituto de Biología Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia –Spain (Phone: +34963877877; Fax: +34963877859; E-mail: [email protected]). * Author for correspondence. T3 pKS+ PBNSPaV 13256 ApLV 13556 8874 9114 1 PLMVd 337 12 HSVd 296 78 PNRSV 257 38 ApMV 208 9295 PPV ACLSV 9518 193 681 110 PDV APLPV 290 102 247 XhoI Schematic representation of the poly10 clone introduced in the pSK+ plasmid. The corresponding virus or viroid fragments are indicated by a box in which the numbers represent the corresponding nucleotide of the data base sequences: EF546442 (PBNSPaV), HQ339956 (ApLV), EU888599 (PLMVd), AJ297832 (HSVd), AJ133201 (PNRSV), AY054388 (ApMV), AB576079 (PPV), AJ586653 (ACLSV), EU169999 (PDV) and EF503724 (APLPV). The XhoI restriction site used either to synthesize the riboprobe with the T7 RNA polymerase or to insert additional cDNA probes is indicated. T7
