Download CV. de Silvia Gómez Sebastián

CURRICULUM VITAE
PERSONAL DATA
PHONE NUMBER:
Silvia Gómez Sebastián
February 18th , 1975
Madrid, Spain
Avd. Badajoz 3, 7 C
28027- MADRID
+34 91 347 3794 (lab)
E-MAIL:
[email protected]
NAME:
BIRTH DATE:
BIRTH PLACE:
HOME ADDRESS:
ACADEMIC BACKGROUND
-1998/2003: PhD, Molecular Biology, Universidad Autónoma of Madrid, Spain.
-2002: member of the Spanish Society for Biochemical and Molecular Biology.
-2000/2001: Master in Molecular Biology, “Severo Ochoa Molecular Biology
Center”. Universidad Autónoma of Madrid. Spain.
-1993/1998: Bachelor Degree in Biology, Biochemistry and Molecular Biology
specialization, University Autonoma of Madrid. Spain.
RESEARCH PROFILE
Research interests: I am interested in Virology from the point of view of its interaction
with hosts (basic Virology) and its application in Biotechnology and Gene Therapy.
1) Employment record
May 2007/present: Alternative Gene Expression (Algenex S.L). Universidad
Politécnica of Madrid Campus of Montegancedo. Pozuelo de Alarcon. Madrid.
Spain. Since 2012 as Head of Research and Exploratory Development (R&D).
Main roles: coordination of the different R&D activities of the company, including:
elaboration of patents and product portfolios and taking active part in jointdevelopments with other biotech companies.
Apr/2006-Apr/2007: Postdoctoral position. Biotechnology Department. INIA.
Madrid. Spain (supervisor: Dr. Jose M. Escribano)
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Main research role: Characterization of relevant immune mechanisms of protection
against African swine fever virus for the development of new vaccine strategies.
Apr/2005-Apr/2006: Postdoctoral fellow. Neurogenetics Department. Welcome
Trust Centre for Human Genetics. Oxford. (UK) (Supervisor: Dr. Wade-Martins).
Main research role: Gene therapy for Friedreich's ataxia using herpesvirus amplicon
vectors.
Jan/2005- March/2005: Postdoctoral fellow. Severo Ochoa Molecular Biology
Department. University Autonoma of Madrid. Spain. (Supervisor: Dr. Filip Lim)
Main research project: Generation of herpesvirus vectors for human Gene Therapy.
Jan/2004- Jan/2005: Postdoctoral position. Department of Medicina Preventiva y
Salud Pública (Microbiología). Faculty of Medicina. University Autonoma of
Madrid. Madrid. Spain. (Supervisor: Prf. Enrique Tabarés).
Main research role: Use of recombinant proteins of pseudorabies virus (PRV) for a
diagnostic assay to differentiate infected from vaccinated animals.
Oct/1998-Dec/2003: Department of Medicina Preventiva y Salud Pública
(Microbiología). Faculty of Medicina. University Autonoma of Madrid. Madrid.
Spain. (Supervisor: Prof. Enrique Tabarés)
Main research roles: 1) Characterization of the negative regulation of herpes simplex
virus type 1 ICP4 promoter by the IE180 protein of pseudorabies virus. 2)
Characterization of recombinant proteins of pseudorabies virus to differentiate infected
from vaccinated animals. 3) Application of pseudorabies virus based-amplicons as
oncolitic and Gene Therapy vectors.
Dec/1997-Oct/1998: Undergraduated research collaborator. Severo Ochoa
Molecular Biology Department. University Autonoma of Madrid. Madrid. Spain.
(Supervisor: Dr. Antonio García Bellido)
Main research role: Analysis of directed-mutagenesis in Drossophila melanogaster.
2) Research projects
Participation in national and international projects
*Diagnostic and vaccination against the new variant of rabbit haemorrhagic disease
virus. (2014-2017)
Grant founded: Ministerio de Economia y Competitividad (130,000 €).
*Development of fluorescent recombinant antibodies to detect African swine fever virus
in tissue samples and infected cells. NPB-11-022 (2012-2013)
Grant founded: Pig producers association. USA (73,000 $)
*Desarrollo de vacunas particuladas derivadas de larvas de insecto (2008-2009)
Grant founded: Comunidad de Madrid (322.273 €)
*Desarrollo de vacunas particuladas derivadas de larvas de insecto como biofactoría y
desarrollo de una nueva línea de productos recombinantes fluorescentes. (2007-2008)
Grant founded: Comunidad de Madrid (304.000 €)
* Desarrollo de nuevas estrategias de control de la peste porcina Africana. (2007-2010)
Grant founded: Ministerio de Educación y Ciencia (160.000 €)
*Patogenia de enfermedades Víricas (PORCIVIR). (2006-2011)
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Grant founded: MEC-CONSOLIDER INGENIO 2010. (4.960.000 €)
*Development of African swine fever virus vaccines and Control of African Swine Fever
Virus (ASFV) through improved understanding of the epidemiology of the disease and
construction and testing of recombinant and attenuated virus vaccines. WT 75813
(2005-2009)
Grant founded: American and Wellcome Trust Foundation.
*Desarrollos biotecnológicos para la utilización rentable de plantas como biofactorías
(2004-2008)
Grant founded: INIA (157.910 €)
*Novel Friedreich's ataxia models and gene therapy strategies using herpesvirus
amplicon vectors in transgenic mice (2004-2006)
Grant founded: Friedreich´s Ataxia Research Alliance (FARA) (70,000 $)
*Aplicación de los amplicones del virus de la pseudorrabia (PRV) en vacunas y terapia
génica (2002-2003)
Grant founded: Comunidad autónoma de Madrid (72.216 €)
*Obtención de las proteínas ORF2.5 y gE del virus de la enfermedad de Aujezsky para
su aplicación al diagnóstico diferencial entre la infección natural y vacunal (1999-2003)
Grant founded: TDI S.A (26.500 €)
Participation in national and international projects as principal investigator
* FP7 : Topic: Prevention of important diseases of farmed fish species, KBBE.2012.1.210. PROFIVAC: this proposal was prepared among seven groups of investigation, four
SME and two big pharma industries, finalized in a second place).
* Horizon 2020: Topic: Tackling losses from terrestrial animal diseases, SFS-1B-2014
APICOVAC. The project has been prepared among 12 research groups, one SME and
a big pharma company. This proposal is currently pending of resolution
3) Publications
patents (Divided in three families)
Nanobodies:
1. Monomeric VHH domain derived from anti-VP6 camelid antibodies, dimeric
domain, immunisation method, rotavirus detection method, composition,
prevention and threatment methods for rotavirus infections. Thomas Surrey,
Aurelien Olichon, Lorena Laura Garaicoechea, Gisela Ariana Marcoppido, Gladys
Viviana Parreño, Silvia Gomez-Sebastian, Jose Angel Martinez Escribano, Andres
Wigdorovitz. PCT/EP2008/059745. Pub. Date: Dec. 3, 2013. Approved. Currently under
extension to: CAN, KR, AU, ZA y SG.
New baculoviral promoters:
2. Insect-derived promoters for foreign proteins expression in insect cells. Silvia
Gomez-Sebastian, Javier Lopez Vidal, Covadonga Alonso Marti, Jose Angel Martinez
Escribano. PCT/EP20080803551. Pub. Date: Oct. 23, 2013. Approved.
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New baculoviral vectors:
3. Recombinant DNA elements for the expression of recombinant proteins in a
host cell.
Silvia Gómez-Sebastián; Javier López-Vidal and Jose Angel Martinez-Escribano.
PCT/EP2012/061081. Pub. Date: Jun 10, 2013
4. Recombinant DNA elements for the expression of recombinant proteins in
insects.
Silvia Gómez-Sebastián; Javier López-Vidal and Jose Angel Martinez-Escribano.
PCT/EP2012/061088. Pub. Date: Jun 10, 2013. Under licensing
5. System Overexpressing baculovirus transactivators for the production of
Recombinant Proteins (IN PARTICULAR, THOSE FORMING VIRUS-LIKE
PARTICLES)
Silvia Gómez-Sebastián; Javier López-Vidal and Jose Angel Martinez-Escribano.
PCT/EP2013/075812. Pub. Date: Jun 12, 2014
6. Enhanced production of the porcine circovirus capsid protein by a baculovirus
vector expression system.
Silvia Gómez-Sebastián; Javier López-Vidal and Jose Angel Martinez-Escribano.
PCT/EP2013/075799. Pub. Date: Jun 12, 2014
Peer-Reviewed Publications (20 publications, h index: 7)
1. Insect larvae biofactories as a low-cost platform for functional Growth Factors
production. Benoit Dudognon, Lorena Romero Santacreu, Silvia Gomez-Sebastian, ML
Bellido, E. Muñoz and J. M. Escribano. J Biotechnol. 2014 Aug 20;184:229-39.
2. Significant productivity improvement of the baculovirus expression vector
system by engineering a novel expression cassette.
Gomez-Sebastian S, Lopez-Vidal J, Escribano JM. PLoS One. 2014. May 13;9(5):e96562.
3. Recombinant monovalent llama-derived antibody fragments (VHH) to rotavirus
VP6 protect neonatal gnotobiotic piglets against human rotavirus-induced
diarrhea.
Vega CG, Bok M, Vlasova AN, Chattha KS, Gómez-Sebastián S, Nuñez C, Alvarado C, Lasa
R, Escribano JM, Garaicoechea LL, Fernandez F, Bok K, Wigdorovitz A, Saif LJ, Parreño V.
PLoS Pathog. 2013 May;9(5):e1003334.
4. Characterization of a Trichoplusia ni hexamerin-derived promoter in the
AcMNPV baculovirus vector.
López-Vidal J*, Gómez-Sebastián S*, Sánchez-Ramos I, Escribano JM.
J Biotechnol. 2013 Jun 10;165(3-4):201-8
*López-Vidal J and S. Gómez-Sebastián have contributed equally to the results presented in this
paper.
5. Head-to-Head Comparison of Three Vaccination Strategies Based on DNA and
Raw Insect-Derived Recombinant Proteins Against Leishmania.
Felicitat Todolí, Alhelí Rodríguez-Cortés, María del Carmen Núñez, Márcia D. Laurenti, Silvia
Gómez-Sebastián, Fernando Rodríguez, Eva Pérez-Martín, José M. Escribano, Jordi Alberola.
(Accepted in PlosOne, October 2012).
6. African swine fever virus serodiagnosis: a general review with a focus on the
analyses of African serum samples.
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Carolina Cubillos*, Silvia Gómez-Sebastian*, Noelia Moreno, María C. Nuñez
Leopold.K.Mulumba-Mfumu, Carlos J.Quembo, Livio Heath, Eric M.C.Etter, Ferran Jori,
Jose.M.Escribano, Esther Blanco. (Accepted in Virus Research, October 2012)
*C. Cubillos and S. Gómez-Sebastián have contributed equally to the unpublished results
presented in this paper.
7. Rotavirus A-specific single-domain antibodies produced in baculovirusinfected insect larvae are protective in vivo.
Gómez-Sebastián S, Nuñez MC, Garaicoechea L, Alvarado C, Mozgovoj M, Lasa R, Kahl A,
Wigdorovitz A, Parreño V, Escribano JM. (2012) BMC Biotechnol. 2012; 12: 59.
8. Antibody recognition of the glycoprotein G of viral haemorrhagic septicemia
virus (VHSV) purified in large amounts from insect larvae.
Encinas, P., Gomez-Sebastian, S., Nuñez, M.C., Gomez-Casado. E., Escribano. J.M., Estepa.
A., and Coll. (2011) BMC Research Notes 4:210.
9. Infectious delivery and long-term persistence of transgene expression in the
brain by a 135 kb iBAC-FRDA genomic DNA expression vector. Alfredo GimenezCassina, Richard Wade-Martins, Silvia Gomez-Sebastian, Juan-Carlos Corona, Filip Lim and
Javier Diaz-Nido. Gene Therapy. (2011) 18(10):1015-9.
10. Insect larvae biofactories as a platform for influenza vaccine production.
Gomez-Casado E, Gomez-Sebastian S, Núñez MC, Lasa-Covarrubias R, Martínez-Pulgarín S,
Escribano JM. Protein Expr Purif. (2011) 79(1):35-43.
11. Humoral and in vivo Cellular Immunity against the Raw Insect-derived
Recombinant Leishmania infantum antigens KMPII, TRYP, LACK and papLe22 in
Dogs from an Endemic Area. Felicitat Todolí, Laia Solano-Gallego, Rafael de Juan, Pere
Morell, Maria del Carmen Núñez, Rodrigo Lasa, Silvia Gómez-Sebastián, José M. Escribano,
Jordi Alberola, Alhelí Rodríguez-Cortés. American Journal of Tropical Medicine & Hygiene.
(2010) 83(6):1287-94.
12. Human papillomavirus-like particles vaccine efficiently produced in a nonfermentative system based on insect larva. Millán AF*, Gómez-Sebastián S*, Nuñez
MC, Veramendi J, Escribano JM. Protein Expr Purif. (2010) 74(1):1-8.
*A. Fernandez-San Millán and S Gómez-Sebastian have equally contributed to this paper.
13. Dynamics and predictive potential of antibodies against insect-derived
recombinant Leishmania infantum proteins during chemotherapy of naturally
infected dogs. Todolí F, Galindo I, Gómez-Sebastián S, Pérez-Filgueira M, Escribano JM,
Alberola J, Rodríguez-Cortés A. Am J Trop Med Hyg. (2010) 82(5):795-800.
14. Immunity conferred by an experimental vaccine based on the recombinant
PCV2 Cap protein expressed in Trichoplusia ni-larvae. Pérez-Martín E, GómezSebastián S, Argilaguet JM, Sibila M, Fort M, Nofrarías M, Kurtz S, Escribano JM, Segalés J,
Rodríguez F. Vaccine. (2010) 8;28(11):2340-9.
15. DnaK/DnaJ-assisted recombinant protein production in Trichoplusia ni larvae.
Mónica Martínez-Alonso*, Silvia Gómez-Sebastián*, José M. Escribano, Juan-Carlos Saiz,
Neus Ferrer-Miralles, Antonio Villaverde. Appl Microbiol Biotechnol. (2010) 86(2):633-9.
*Mónica Martínez-Alonso and Silvia Gómez-Sebastián have equally contributed to this paper.
16. Seroreactivity against raw insect-derived recombinant KMPII, TRYP, and
LACK Leishmania infantum proteins in infected dogs. Felicitat Todolı, Mariano PerezFilgueira, Inmaculada Galindo, Silvia Gomez-Sebastian, Jose M. Escribano, Alhelı RodrıguezCortes, Jordi Alberola. Veterinary Parasitology. (2009) 164(2-4):154-161.
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17. Development of two Trichoplusia ni larvae-derived ELISAs for the detection of
antibodies against replicase and capsid proteins of porcine circovirus type 2 in
domestic pigs. Eva Pérez-Martína, Llorenc¸ Grau-Romaa, Jordi M. Argilaguet, Miquel
Nofrarías, José M. Escribano, Silvia Gómez-Sebastián, Joaquim Segalés, Fernando
Rodríguez. Journal of Virological Methods. (2008) 154 (1-2): 167-174.
18. DIVA diagnostic of Aujeszky’s disease using an insect-derived virus
glycoprotein E. S. Gómez-Sebastián, D.M. Pérez-Filgueira, E. Gómez-Casado, M.C.
Nuñez, I. Sánchez-Ramos, E. Tabarés, J.M. Escribano. Journal of Virological Methods.
(2008). 153 (1): 29-35.
19. Infectious delivery and expression of a 135 kb human FRDA genomic DNA
locus complements Friedreich's ataxia deficiency in human cells. Silvia GomezSebastian, Alfredo Gimenez-Cassina, Javier Diaz-Nido, Filip Lim and Richard WadeMartins. Molecular Therapy.(2007) 15 (2): 248-254.
20. Negative regulation of herpes simplex type 1 ICP4 promoter by IE180 protein
of pseudorabies virus. S. Gómez-Sebastián, E. Tabarés. Journal of General
Virology. (2004) 85: 2115-2130.
Manuscripts submitted or in preparation
1. Expression and characterization of the gD protein of HSV-2 fused to the
tetramerization domain of the transcription factor p53. M. Pérez-Hernández; I.
Gadea; J.M. Escribano; E. Tabarés1 and S. Gómez-Sebastián*. Submitted
Protein Expression and Purification. Jan 2014
2. Unleashing the potential of BEVS-based technologies. S. Gómez-Sebastián*.
Submitted
Critical Review
OA Biotechnology. Jan 2014
3. Highly efficient insect cell-based platform for virus-like particle vaccines
production using an improved baculovirus vector
Javier López-Vidal*, Silvia Gómez-Sebastián*, Juan Bárcena, Maria C. Nuñez, Diego MartínezAlonso, Eva Guijarro Pardo, Benoit Dudognon and José M. Escribano.*equal contribution
4. Improved recombinant protein production in insect-based biofactories by
using baculovirus vectors genetically engineered by a novel expression
cassette
Silvia Gómez-Sebastián*, Javier López-Vidal*, Edel Reytor, Miguel Cid, Carmen Alvarado,
Lorena Romero Santacreu, Maria C. Nuñez and José M. Escribano.*equal contribution
4) Conference comunications (2013-2014)
- FEBS-EMBO Congress. 30 August-Paris, France from 30 August - 4 September
(2014). Paris. France. Improved baculovirus vector as high-productive expression
system for developing a subunit vaccine against Influenza. Eva Guijarro-Pardo*,
Silvia Gómez-Sebastián*, Martínez-Pulgarín Susana, Escribano J.M.* Equal
contribution.
- ANQUE-ICCE-BIOTEC2014. July 1-4 (2014). Madrid. Spain. Highly efficient insect
cell-based platform for virus-like particle vaccines production using an improved
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baculovirus vector J. López-Vidal, S. Gómez-Sebastián, J. Bárcena, M.C. Nuñez, D.
Martínez-Alonso, B. Dudognon, E. Guijarro-Pardo and J.M. Escribano
- XXXVI Congress of the Biochemical and Molecular Biology Society (Spanish Society
for Molecular Biology). September 3-6 (2013). Madrid. Spain Proliferative and cell
protective effects of the AcMNPV IE0 and IE1 proteins when are over-expressed
in a baculovirus vector context. Javier López Vidal, Eva Guijarro Pardo, Silvia
Gómez-Sebastián, José M. Escribano
- XXII National Congress of Virology. June 9-12 (2013). Burgos (Spain). Definition of
regulatory genetic elements of a baculovirus expression cassette which
significantly improves the production characteristics of the BEVS. GómezSebastián S, López-Vidal J, M.C. Nuñez, E.G. Pardo, Escribano J.M.
5) Other scientific achievements
Review in Process Biochemistry in 2014.
Review in Expert Review of Molecular Diagnostics in 2010.
Registered as expert in the Horizon 2020 program. Expert id: EX2006C165452.
6) Organization membership
Membership of the Sociedad Española de Bioquimica y Biología Molecular (SEBBM)
since 2002. This year (2014) I have been promoted to ordinary membership due to my
research progression and achievements.
7) Awards and Fellowships
Contract: Torres Quevedo. Desarrollo de un nuevo sistema de diagnóstico serológico
basado en proteínas fluorescentes recombinantes producidas en larvas de insecto.
(2007-2010), Funding: Spanish Government.
Fellowship: Severo Ochoa Fundation. 3 months (2005)
8) Technical skills
Basic Molecular Biology: DNA and RNA extraction, PCR, RT-PCR, cloning,
transfection, transformation, transduction, immunofluorescence, western blot, protein
expression and purification, cell culture, Southern blot, Northern blot, Flow cytometry.
Virology: herpesvirus manipulation, baculovirus manipulation, titration, clonal selection.
DNA extraction, viral purification.
Biocomputation: DNA programs (Ape, Chromas), DNA database (Genebank), DNA
sequence analysis (nucleotide blast, megablast), proteomic tools (proteomic blast,
Signal IP, TargetP, ProtScale).
TEACHING PROFILE
1) Teaching record
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2014-2015. Honorific teacher. Department of Medicina Preventiva y Salud Pública
(Microbiología). Faculty of Medicine. University Autonoma of Madrid. Madrid. Spain.
2009-2013. Speaker. Master of Viral Biotechnology. Faculty of Medicine. University
Autonoma of Madrid. Madrid. Spain.
2004-2005. Postgraduate research teaching. Department of Medicina Preventiva y
Salud Pública (Microbiología). Faculty of Medicine. University Autonoma of Madrid.
Madrid. Spain.
2002-2003. Collaborator at Virology practices. Subject: Microbiology
Parasitology. Faculty of Medicine. University Autónoma of Madrid. Madrid. Spain.
and
2000 and 2002. Lab teaching assistant. Subject: Gene Expression I practices. VI and
V Master of Biotechnology. Severo Ochoa Molecular Biology Department. University
Autonoma of Madrid. Spain.
2000-2001. Teaching assistant. Elective subject: Initiation to the Scientific Method for
Medicine. Faculty of Medicine. University Autonoma of Madrid. Madrid. Spain.
1999/2000. Teaching assistant Subject: Experimental Biochemistry practices.
Biochemistry. Faculty of Science. University Autonoma of Madrid. Madrid. Spain.
2) Mentoring
co-director for graduate students: Eva Guijarro Pardo (2011), Marta Pérez-Hernández
(2011)
Co-director of Ph.D. sudents: Javier López-Vidal (2009-2014) University Complutense
of Madrid. Eva Guijarro Pardo (2011-present) University Autonoma of Madrid, Benoit
Dudognon (2010-2014) pending of defense.
3) Other achievements
In June 2014 I have achieved the accreditation for Full, Associate and Assistant
Lecturers from the Agencia Nacional de Evaluación de la Calidad y Acreditación
(ANECA)
4) Languages (A = Fluent, B = Good, C = Basic)
Spanish (A), English (A-B)
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