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6th International Geminivirus Symposium & th 4 International ssDNA Comparative Virology Workshop PROGRAM Guanajuato, Guanajuato, México November 7 – 12, 2010 6th International Geminivirus Symposium i Editors Committee Organizing Committee National Rafael Rivera-Bustamante Diana L. Trejo Saavedra Luis Pérez Moreno Irineo Torres-Pacheco Ramón Guevara-González Oscar Moreno Valenzuela Jesús Méndez-Lozano J.A. Garzón-Tiznado Rodolfo de la Torre-Almaraz Gerardo Argüello-Astorga Raúl Díaz-Plaza Jaime Holguín-Peña Organizing Committee International J.T. Ascencio-Ibañez Linda Hanley Bowdoin Claude Fauquet Judith K. Brown F. Murilo Zerbini Ed Rybicki Eduardo R. Bejarano Rob Briddon Gian Paolo Accotto Philippe Biagini Xian-Jim Meng Peter Tijssen Siobain Duffy ii 4th International ssDNA Comparative Virology Workshop 6th International Geminivirus Symposium 4 International ssDNA Comparative Virology Workshop November 7 – 12, 2010 Guanajuato, México th Welcome to Guanajuato: Three years ago en Ouro Preto, Brazil, you decided to come to Mexico. During the presentation I felt that the slide that was most appreciated was: “Why Mexico? Because we want you there!”. Indeed, we are very happy that you are coming to Guanajuato, Mexico for the 6th International Geminivirus Symposium and the 4th International ssDNA comparative virology Workshop. Welcome. What is the formula, the recipe to make a scientific meeting a success? The first and key ingredient that has been always present in the previous Symposia: an excellent academic program. And that means you. We have registered more than 150 attendants from all around the World, we have scheduled more than 30 keynote / plenary talks and have received more than 100 abstracts. The contributions received for this meeting reflect interesting, informative major tendencies on ssDNA virology research. Virus-host interaction and virus diversity / evolution are favorite research areas this year. We will be presented also with new methodologies and technological platforms that are helping us to generate more data than ever, that facilitate new discoveries. In my opinion this is excellent news. Yes, we are characterizing new viruses, detecting new pathogenic entities, and learning how these pathogens evolve, and interact among themselves But we are also learning like never before how these entities interact with the host cells, with their machinery, and at the end, how they cause disease. Which is probably what the general population is interested in. As in many other scientific disciplines, bioinformatics is becoming a major player in our research, not only to analyze and compare the now hundreds of virus genomes, but also how to assemble new genomes without cloning them, how to trace back an important pathogen to its origins, and even how to understand the meaning of hundreds of genes that are over- or under-expressed in a infected host cell. Finally, another learning experience from analyzing geminivirus literature is that research has become more and more an interdisciplinary and collaborative activity. We hope that this meeting will be “the origin”, “the source”, “a melting-pot”, for new and productive collaborations that will benefit everybody. To those with years of experience, let us show the students, those that represent the next generations, the advantages of collaboration and coordinated efforts. That can be also an important legacy. Enjoy Guanajuato and remember this is also a Birthday Party. Rafael Rivera-Bustamante For the organizing committee. 6th International Geminivirus Symposium iii Funding Institutions iv 4th International ssDNA Comparative Virology Workshop Sponsors 6th International Geminivirus Symposium v Table of Contents Schedule .......................................................................................................... viii Program of Events Sunday .................................................................................................... 3 Monday ................................................................................................... 4 Tuesday .................................................................................................. 7 Wednesday ..................................................................................................... 10 Thursday ......................................................................................................... 12 Friday .............................................................................................................. 15 Poster Sessions ................................................................................................ 19 Author Index ................................................................................................... 45 vi 4th International ssDNA Comparative Virology Workshop 14:00 TO 18:30 REGISTRATION SUNDAY NOV. 7 PLENARY SESSION 3 XUEPING ZHOU PLENARY SESSION 3 14:00 – 14:30 PLENARY SESSION 5 MYA BREITBART PLENARY SESSION 5 GIAN PAOLO ACCOTTO WORKSHOP TALKS VIRUS HOST INTERACTION II WORKSHOP TALKS EMERGING / NOVEL VIRUSES LUNCH 12:30 – 14:00 14:00 – 16:00 LUNCH PLENARY SESSION 4 CLAUDE FAUQUET 12:00 – 12:30 11:30 – 12:00 PLENARY SESSION 4 CHRISTINA WEGE PLENARY SESSION 2 SONDRA LAZAROWITZ PLENARY SESSION 2 PETER TIJSSEN 10:30 – 11:00 H. JOSEF VETTEN PLENARY SESSION 1 DAVID BISARO PLENARY SESSION 1 PHILIPPE BIAGINI 11:00 – 11:30 COFFEE BREAK LINDA HANLEY-BOWDOIN KEYNOTE SPEAKER COFFEE BREAK EDUARDO R. BEJARANO KEYNOTE SPEAKER PLENARY TALKS PLENARY TALKS VIRUS HOST INTERACTION I PLENARY TALKS RECENT ADVANCES IN SSDNA RESEARCH SPECIAL SSDNA TAXONOMY WORKSHOP FREE AFTERNOON LUNCH PLENARY SESSION 4 TBA PLENARY SESSION 3 BRUNO GRONENBORN PLENARY SESSION 5 CLAUDE FAUQUET WORKSHOP TALKS VIRUS DIVERSITY AND EVOL LUNCH PLENARY SESSION 4 DARREN MARTIN PLENARY SESSION 3 F MURILO ZERBINI PLENARY SESSION 2 ROB BRIDDON PLENARY SESSION 1 JUDITH K BROWN PLENARY SESSION 1 ELIZABETH FONTES PLENARY SESSION 2 SUNIL K MUKHERJEE COFFEE BREAK VIRUS DIVERSITY/ EVOLUTION HOLGER JESKE KEYNOTE SPEAKER PLENARY TALKS BREAKFAST THURSDAY NOV. 11 COFFEE BREAK ETHEL-MICHELE DE VILLIERS KEYNOTE SPEAKER VIRUS HOST INT. II BREAKFAST BREAKFAST WEDNESDAY NOV. 10 BREAKFAST TUESDAY NOV. 9 10:00 – 10:30 9:00 – 10:00 7:30 – 9:00 MONDAY NOV. 8 LUNCH NEXT MEETING PRESENTATION AND CLOSING REMARKS 10:30 – 12:30 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS COFFEE BREAK 9:30 – 10:00 PLENARY SESSION 2 J C FARIA 9:00 – 9:30 PLENARY SESSION 1 ENRIQUE MORIONES DISEASE, EPID. AND DISEASE CONTROL PLENARY TALKS BREAKFAST FRIDAY NOV. 12 6th International Geminivirus Symposium, November 7-12, 2010. Guanajuato, Mexico 18:30 TO 20:30 WELCOME COCTAIL SUNDAY NOV. 7 21:00 18:30 – 20:00 17:00 – 18:15 16:30 – 17:00 POSTER SESSION EVEN NUMBERS CALLEJONEADA FOLKLORIC BALLET 17:30 – 18:15 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS 17:00 – 17:30 PLENARY SESSION 8 MIKHAIL POOGGIN PLENARY SESSION 7 GARRY SUNTER VIRUS REPLICATION – GENE EXP. WORKSHOP TALKS POSTER SESSION ODD NUMBERS ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS PLENARY SESSION 7 DOMINIQUE ROBERTSON WORKSHOP TALKS VIRUS HOST INTERACTION I COFFEE BREAK 16:00 – 16:30 16:30 – 18:30 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS 15:00 – 15:45 COFFEE BREAK RAFAEL RIVERA-BUSTAMANTE PLENARY SESSION 6 TUESDAY NOV. 9 PLENARY SESSION 6 ARVIND VARSANI 14:30 – 15:00 MONDAY NOV. 8 WEDNESDAY NOV. 10 GALA DINNER 16:30 – 18:15 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS VIRUS VIRUS DIV. / EVOL. WORKSHOP TALKS COFFEE BREAK ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS PLENARY SESSION 6 SIOBAIN DUFFY THURSDAY NOV. 11 FRIDAY NOV. 12 6th International Geminivirus Symposium, November 7-12, 2010. Guanajuato, Mexico PROGRAM OF EVENTS Exit Sunday November 7, 2010 ________________________________________________________________________________________________________________________________________________ 14:00 to 20:00 Registration San Gabriel Room 18:30 – 20:30 Welcome Cocktail All oral presentations will be held in the La Mancha Room All poster presentations will be held in the La Mancha Room 6th International Geminivirus Symposium 3 Exit Monday November 8, 2010 _____________________________________________________________________________________________________________________________________________ Plenary Talks Recent Advances in ssDNA Research ________________________________________________________________________________________________________________________________________________ 9:00 – 10:00 Keynote Address Eduardo Bejarano IHSM – UMA – CSIC, Spain Geminiviral avoidance and exploitation of the posttranslational systems 10:00 – 10:30 Coffee Break 10:30 – 11:00 Philippe Biagini Université de la Méditerranée, France Taxonomy of Anelloviridae and Circoviridae: past, present, future 11:00 – 11:30 Peter Tijssen Université du Québec, Canada Parvoviridae: small viruses, big surprises 11:30 – 12:00 H. Josef Vetten Julius Kühn Institute, Germany Recent advances in the study of viruses of the family Nanoviridae 12:00 – 12:30 Claude Fauquet ILTAB – Danforth Center Ever increasing biodiversity of single stranded DNA viruses 12:30 – 14:00 Lunch 4 4th International ssDNA Comparative Virology Workshop Exit Monday November 8, 2010 ________________________________________________________________________________________________________________________________________________ Workshop Talks Emerging / Novel Viruses 14:00 – 14:30 Mya Breitbart - Plenary University of South Florida, USA Discovery of Novel Plant and Animal Viruses Through Vector-Enabled Metagenomics on Whiteflies and Mosquitoes 14:30 – 15:00 Arvind Varsani - Plenary University of Canterbury, University of Cape Town, South Africa Gemini-r-evolution: Discovery of novel viruses and insights into the interplay between viruses infecting cultivated and indigenous plants 15:00 – 15:15 Huma Muntaz NIBGE – Pakistan Diversity of Dicot-infecting Mastreviruses 15:15 – 15:30 Karyna Rosario University of South Florida, USA Discovery of a novel single-stranded DNA virus in dragonflies 15:30 – 15:45 Cecilia Hernández The University of Arizona, USA Characterization of two newly discovered curtoviruses isolated from spinach in south-central Arizona 15:45 – 16:00 Jesús Navas-Castillo IHSM – UMA – CSIC, Spain Genetic diversity of DNA satellites associated with Ipomoeainfecting monopartite begomoviruses 16:00 – 16:30 Coffee Break 6th International Geminivirus Symposium 5 Exit Monday November 8, 2010 ___________________________________________________________________ Workshop Talks Virus Host Interaction I ___________________________________________________________________ 16:30 – 17:00 Dominique Robertson - Plenary North Carolina State University, USA Gemini-VIGS in cotton 17:00 – 17:15 Bernardo Bañuelos IPICyT, Mexico Two strains of Euphorbia mosaic virus are incompatible in replication and contain short Rep sequences in the BV1 promoter region 17:15 – 17:30 Raúl Álvarez-Venegas CINVESTAV – IPN Irapuato, Mexico Use of Virus-Induced Gene Silencing (VIGS) to accelerate genetic gain 17:30 – 17:45 Asako Uchiyama Cornell University, USA Arabidopsis synaptotagmins: their roles in virus cell-to-cell movement and plant development 17:45 – 18:00 J. Trinidad Ascencio-Ibañez North Carolina State University, USA BCTV and CaLCuV display a different interaction with the cell cycle machinery in Arabidopsis 18:00 – 18:15 Jyothna P Indian Agricultural Research Institute, India Characterization of recovery phenomenon in Tomato leaf curl New Delhi virus : Correlation between viral DNA, transcripts and miRNA levels 18:30 – 20:00 Poster Session (Odd-numbered posters) 6 4th International ssDNA Comparative Virology Workshop Exit Tuesday November 9, 2010 _____________________________________________________________________________________________________________________________________________ Plenary Talks Virus Host Interaction ________________________________________________________________________________________________________________________________________________ 9:00 – 10:00 Keynote Address Linda Hanley-Bowdoin North Carolina State University, USA Dissecting geminivirus/host interactions in Arabidopsis 10:00 – 10:30 Coffee Break 10:30 – 11:00 David Bisaro The Ohio State University, USA RNA-directed methylation targeted to geminivirus genomes: Defense and counter-defense 11:00 – 11:30 Sondra Lazarowitz Cornell University, USA Plant cell-to-cell trafficking: Viruses touch a nerve 11:30 – 12:00 Xueping Zhou Zhejiang University, P R China Tomato SlSnRK1 protein interacts with and phosphorylates geminivirus βC1 protein to attenuate viral infection 12:00 – 12:30 Christina Wege University of Stuttgart, Germany Inter-species complementation of Old and New World begomoviruses in spread: "Artificial" two-component infections dissect virus-plant-interactions 12:30 – 14:00 Lunch 6th International Geminivirus Symposium 7 Exit Tuesday November 9, 2010 ________________________________________________________________________________________________________________________________________________ Workshop Talks Virus Host Interaction II 14:00 – 14:30 Gian Paolo Accotto - Plenary Istituto di Virologia Vegetale – C.N.R, Italy Transcriptomic analysis of tomato infected by Tomato yellow leaf curl Sardinia virus 14:30 – 15:00 Rafael Rivera-Bustamante - Plenary CINVESTAV – IPN Unidad Irapuato, Mexico Characterization of a recovery stage in geminivirus-infected pepper plants: Transcriptomic analysis 15:00 – 15:15 Imran Amin NIBGE - Pakistan Four suppressors of RNA silencing are encoded by cotton leaf curl Multan begomovirus complex 15:15 – 15:30 Muhammad Mubin University of Agriculture Faisalabad, Pakistan The precoat protein encoded by a monopartite begomovirus is a suppressor ofboth post transcriptional and transcriptional gene silencing 15:30 – 15:45 Edgar Rodríguez-Negrete IHSM, UMA-CSIC, Spain The Tomato yellow leaf curl virus, Sardinian isolate (TYLCSV) C4 protein is a putative transcriptional gene silencing (TGS) suppressor 15:45 – 16:00 Pradeep Sharma Directorate of Wheat Research, India The V2 protein of Monopartite begomovirus determines virulence, hypersensitive response and suppression of posttranscriptional gene silencing 16:00 – 16:30 Coffee Break 8 4th International ssDNA Comparative Virology Workshop Exit Tuesday November 9, 2010 ___________________________________________________________________ Workshop Talks Virus Replication – Gene Expression ___________________________________________________________________ 16:30 – 17:00 Garry Sunter - Plenary The University of Texas, USA Functional studies of bipartite complementary sense promoters 17:00 – 17:15 Mikhail Pooggin - Plenary University of Basel, Switzerland Primary and secondary siRNAs in geminivirus induced gene silencing 17:15 – 17:30 Björn Krenz University of Stuttgart, Germany The impact of geminiviral proteins on the nuclear architecture 17:30 – 17:45 Aurora Londoño IPICyT, Mexico Identification of replication specificity determinants in eukaryotic ssDNA viruses 17:45 – 18:00 Poliane Alfenas-Zerbini Universidade Federal de Viçosa, Brazil APC7, a component of the Anaphase promoting complex, in begomovirus replication. 18:30 – 20:00 Poster Session (Even-numbered posters) 21:00 Callejoneada 6th International Geminivirus Symposium 9 Exit Wednesday November 10, 2010 _____________________________________________________________________________________________________________________________________________ Plenary Talks Virus Host Interaction II / Replication of ssDNA viruses ________________________________________________________________________________________________________________________________________________ 9:00 – 10:00 Keynote Address Ethel-Michele de Villiers Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Germany The diversity of TT viruses: In vitro replication leads to the formation of virus-like particles and additional replication competent subviral molecules 10:00 – 10:30 Coffee Break 10:30 – 11:00 Elizabeth B. Fontes Universidade Federal de Viçosa, Brazil NSP-interacting kinase, NIK: a transducer of plant defense signaling suppressed by the begomovirus nuclear shuttle protein 11:00 – 11:30 Sunil K. Mukherjee Plant Mol. Biol. Group, ICGEB, India Role of RAD54 protein in geminivirus DNA replication 11:30 – 12:00 Bruno Gronenborn Institut des Sciences du Végétal, CNRS, France Variability and Evolution Rate of Plant Viruses with a SingleStranded DNA Genome 12:30 – 14:00 Lunch Free Afternoon 10 4th International ssDNA Comparative Virology Workshop Exit Wednesday November 10, 2010 ________________________________________________________________________________________________________________________________________________ Special ssDNA Taxonomy Workshop 15 minutes presentations Tax 1 A possible new taxonomy for the family Geminiviridae Claude Fauquet Tax 2 Divergent monopartitite geminiviruses: Eragrostis curvula streak virus and Turnip curly top virus Arvind Varsani Tax 3 A new species threshold for Mastreviruses Claude Fauquet Tax 4 A new taxa to accommodate two new “Curtovirus” species from Iran Arvind Varsani Tax 5 A new subgenus to accommodate the Swepoviruses Jesus Navas-Castillo and Judith K. Brown Tax 6 A new subgenus to accommodate the Legumoviruses and Copaviruses Claude Fauquet Tax 7 Alphasatellite taxonomy Rob Briddon, Claude Fauquet Tax 8 Betasatellite taxonomy Rob Briddon, Claude Fauquet Tax 9 Circovirus species threshold Arvind Varsani 6th International Geminivirus Symposium 11 Exit Thursday November 11, 2010 _____________________________________________________________________________________________________________________________________________ Plenary Talks Virus Diversity and Evolution ________________________________________________________________________________________________________________________________________________ 9:00 – 10:00 Keynote Address Holger Jeske Universität Stuttgart, Germany Circomics of geminiviruses 10:00 – 10:30 Coffee Break 10:30 – 11:00 Judith K. Brown The University of Arizona, USA The contribution of an alphasatellite-like molecule to symptom severity when in the presence of a begomovirus-beta satellite complex infecting tomato in Oman 11:00 – 11:30 Rob W. Briddon NIBGE - Pakistan Betasatellites - then and now" 11:30 – 12:00 F. Murilo Zerbini Universidade Federal de Viçosa, Brazil From 'wild' to 'domesticated' and back to 'wild': Genetic diversity and evolution of Brazilian begomoviruses 12:00 – 12:30 Darren Martin University of Cape Town, South Africa Constraints on the adaptive value of recombination in ssDNA viruses 12:30 – 14:00 Lunch 12 4th International ssDNA Comparative Virology Workshop Exit Thursday November 11, 2010 ________________________________________________________________________________________________________________________________________________ Workshop Talks Virus Diversity and Evolution 14:00 – 14:30 Claude Fauquet - Plenary ILTAB, USA High diversity and high infectiosity of geminiviruses and their associated satellites in symptomless reservoirs plants 14:30 – 15:00 Siobain Duffy - Plenary Rutgers University, USA How do ssDNA viruses evolve as quickly as RNA viruses? 15:00 – 15:15 Elvira Fialló-Olivé Centro Nacional de Sanidad Agropecuaria, Cuba Begomovirus diversity and recombination in the Caribbean, a hot 'melting pot' in the New World 15:15 – 15:30 Radha Anandalakshmi Mahyco Research Center, India Veni Vidi Vici - The tale of the Cotton leaf curl Burewala virus in India 15:30 – 15:45 M. M. S. Almeida Universidade de Brasília / Embrapa Vegetables, Brazil Malvaceous plants are infected with novel Begomovirus species 15:45 – 16:00 Jane Polston University of Florida, USA Characterization of a new virus discovered by vector-enabled metagenomics on the whitefly vector 16:00 – 16:30 Coffee Break 6th International Geminivirus Symposium 13 Exit Thursday November 11, 2010 ___________________________________________________________________ Workshop Talks Virus Diversity and Evolution ___________________________________________________________________ 16:30 – 16:45 Moshe Lapidot Volcani Center, Israel Use of rolling circle amplification to enable rapid completion of Koch’s postulates for circular DNA viruses 16:45 – 17:00 Supriya Chakraborty Jawaharlal Nehru University, India Genetic diversity and synergistic interaction among newly emerging begomoviruses associated with chilli leaf curl disease in India 17:00 – 17:15 A. L. Monjane University of Cape Town, Observatory, South Africa Genetic analysis and phylogeographic history of MSV reveals the spatial and temporal origins of contemporary MSV-A recombinant lineages and identifies southern Africa as the ultimate origin of Maize streak disease 17:15 – 17:30 Gordon W. Harkins South African National Bioinformatics Institute The spread of Tomato yellow leaf curl virus (TYLCV) from the Middle East to the world 17:30 – 17:45 L. L. Esterhuizen University of Johannesburg, South Africa Epidemiology and molecular characterization of Tomato curly stunt virus and its insect vector Bemisia tabaci in South Africa 17:45 – 18:00 T. G. Pereira Universidade de Brasília / Embrapa Vegetables, Brazil Begomovirus species diversity in South American weeds 21:00 14 Gala Dinner Sponsored by AgroBio-Mexico 4th International ssDNA Comparative Virology Workshop Exit Friday November 12, 2010 _____________________________________________________________________________________________________________________________________________ Plenary Talks Disease, Epidemiology and Disease Control ________________________________________________________________________________________________________________________________________________ 9:00 – 9:30 Enrique Moriones - Plenary IHSM-UMA-CSIC, Spain Integrated management of tomato yellow leaf curl disease 9:30 – 10:00 J. C. Faria Embrapa Arroz e Feijão, Brazil The epidemiology of Bean golden mosaic virus in two transgenic bean lines 10:00 – 10:30 Coffee Break 10:30 – 10:45 L. Diniz-Mendez Instituto Oswaldo Cruz, FIOCRUZ, Brazil Assessment of human torque teno virus removal from two wastewater treatment plants 10:45 – 11:00 Eybishtz Assaf The Hebrew University of Jerusalem, Israel A reverse genetics approach to discover tomato genes underlying resistance to TYLCV 11:00 – 11:15 R. C. Pereira-Carvalho CNPH, Embrapa Vegetable Crops, IHSM, Brazil Segregation pattern of genetic resistance to Tomato yellow leaf curl virus - Israel (TYLCV-IL) in F2:3 families derived from the tomato hybrid ‘TyKing’ 11:15 – 11:30 Orlene Guerra-Peraza North Carolina State University, USA Quantitative trait loci analysis and whole genome sequences as tools to decipher the resistance to CaLCuV in the Pla-1 ecotype of Arabidopsis thaliana 6th International Geminivirus Symposium 15 Exit 11:30 – 11:45 I. Levin The Volcani Center, Israel Molecular dissection of Ty-5, a Tomato yellow leaf curl virus resistance locus in the tomato line TY172 derived from Solanum peruvianum 11:45 – 12:00 Ligia M. Almeida Embrapa Recursos Genéticos e Biotecnologia, Brazil RNAi-resistance to bipartite begomoviruses in genetically modified tomatoes 12:00 – 12:15 Daisy Stainton University of Canterbury, New Zealand Genetic diversity of Banana bunchy top virus (BBTV) in the South Pacific Tongan archipelago 12:30 16 NEXT MEETING PRESENTATIONS AND CLOSING REMARKS 4th International ssDNA Comparative Virology Workshop POSTER SESSIONS Exit POSTER SESSION ANELLOVIRIDAE Epidemiology 1 Genogroup distribution and viral load of Torquetenovirus in fecal samples. C. A. Pinho-Nascimento1, J. P. G. Leite2, C. Niel1, L. Diniz-Mendes1 1 Laboratório de Virología Molecular; 2Laboratório de Virología Comparada e Ambiental, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil 4365, 21040-360 Rio de Janeiro, RJ, Brasil Replication 2 Transcription of TTV-HD types during in vitro virus replication. Jian-Wei Fei, Romana Kimmel and Ethel-Michele de Villiers Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany 3 Stimulation of TT replication by EBV in cells of lymphatic origin. Silvia S. Borkosky, Corinna Whitley, Annette Kopp-Schneider, Harald Zur Hausen and Ethel-Michele de Villiers Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany Other 4 Assessment of human torque teno virus removal from two wastewater treatment plants. L. Diniz-Mendes1, C.A. Pinho-Nascimento1, L.R. Bergsten-Torralba1, V.S. De Paula, M.P. Miagostovich2 and C. Niel1 1 Laboratório de Virologia Molecular; 2Laboratório de Virologia Comparada e Ambiental; Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, FIOCRUZ – Avenida Brasil, 4365 – CEP21040-360 Rio de Janeiro, RJ, Brasil. 3 CIRCOVIRIDAE Genetic Diversity/Evolution 5 Origin and evolution of porcine circovirus type 2 in Cuba. 6th International Geminivirus Symposium 19 Exit POSTER SESSION Lester Josué Pérez, Heidy Díaz de Arce, Martí Cortey, Patricia Domínguez, Maria I. Percedo, Carmen Laura Perera, Joan Tarradas, Maria T. Frías, Joaquim Segalés, LLilianne Ganges, José Ignacio Núñez Centro Nacional de Sanidad Agropecuria (CENSA), La Habana, Cuba 6 Discovery of a novel single-stranded DNA virus in dragonflies. K. Rosario1, M. Marinov2, D. Stainton2, S. Kraberger2, D. Martin3, M. Breitbart1, and A. Varsani2 1 College of Marine Science, University of South Florida, St. Petersburg, FL, USA, 2School of Biological Sciences, University of Canterbury, Chirstchurch, New Zealand, 3Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rondebosch, South Africa 7 Identification of replication specificity determinants in eukaryotic ssDNA viruses A. Londoño, L. Riego-Ruiz, G.R. Argüello-Astorga IPICyT, Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P. 78216 GEMINIVIRIDAE Taxonomy/Emerging viruses 8 Molecular characterization and phylogenetic begomoviruses infecting weeds in Brazil. relationships of two novel A.T.M. Lima1, D.R. Barros1, F.N. Silva1, P. Alfenas-Zerbini1, C.S. Rocha1, E.W. Kitajima2 and F.M. Zerbini1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570000, Brazil; 2NAP/MEPA, ESALQ-USP, Piracicaba, SP, 13418-900, Brazil 9 Begomoviruses infecting weeds in Bolivia A.T.M. Lima1, F.N. Silva1, C.S. Rocha1, T.F.S. Antunes1, D.R. Barros1, G.P. Rosales2, N. Ortuño2, A. Gandarillas2, R.O. Resende3 and F. M. Zerbini1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil; Fundación PROINPA, Av. Meneces s/n. Km. 4, Zona El Paso, Cochabamba, Bolivia; 3Dep. de Biologia Celular/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, 70910-900, Brazil 2 10 Malvaceous plants are infected with novel Begomovirus species. 20 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION M.M.S. Almeida1,2, L.V. Hoffmann3, J.C. Barbosa4, S.A. Aranha1,2, and A.K. InoueNagata1,2 1 Universidade de Brasília, 70910-900 Brasília, Brazil; 2Embrapa Vegetables, BR 060, Km 09, C.P. 218, 70359-970, Brasília, Brazil; 3Embrapa Algodão, GO 462, Km 12, zona rural, C.P. 179, 75375000, Santo Antônio de Goiás, Brazil; 4ESALQ/USP – Departamento de Fitopatologia e Nematologia, Piracicaba, Brazil 11 Beak and feather disease virus: Global genetic diversity, geographical and host species distribution of isolates. Arvind Varsani1,2, Guy L. Regnard3, Inga I. Hitzeroth3, Robert R. Bragg4, Edward P. Rybicki3,5 1 School of Biological Sciences, University of Canterbury, Christchurch, 8140, New Zealand, 2 Electron Microscope Unit, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa, 3 Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa, 4 Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, 9301, South Africa, 5Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa 12 Molecular characterization of two new strains of Okra yellow mosaic Mexico virus. Y. Cárdenas-Cornejo, M.Cantú-Iris, S. Ambriz-Granados, and G. Argüello-Astorga Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P. 78216 13 Genetic diversity and synergistic interaction among newly emerging begomoviruses associated with chilli leaf curl disease in India. S. Chakraborty, A. K. Singh, R. Vinothkumar, N. Khushwaha, B. Chattopadhyay Molecular Virology laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi – 110 067 14 Molecular characterization of a novel begomovirus infecting the weed Corchorus hirtus in Brazil. R. S. Fontenele1, 2, R. Blawid1, 2, S. G. Ribeiro 1 1 Embrapa Recursos Genéticos e Biotecnologia, Pq Estação Biológica, Brasília, Brazil, 2Universidade de Brasília, Instituto de Biologia, Brasília, Brazil 15 Characterization of two newly discovered curtoviruses isolated from spinach in south-central Arizona. Cecilia Hernández-Zepeda1, Gerardo Arguello-Astorga2, and Judith K. Brown1 6th International Geminivirus Symposium 21 Exit POSTER SESSION 1 School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ USA; 2Division de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica,, A.C. San Luis Potosí, México 16 TYLCV in Northern Mexico, an emerging viral disease of tomato crops in single or mixed infection with natives begomovirus. Méndez-Lozano J., Romero-Romero, J.L., Camacho-Beltrán E., Orduño-Vega, W., GámezJiménez, C., Magallanes-Tapia, M.A., and Leyva-López, N.E. Departamento de Biotecnología Agrícola, CIIDIR Unidad Sinaloa del Instituto Politécnico Nacional. Blv. Juan de Dios Bátiz Peredes 250, C.P. 81101. Guasave, Sinaloa, México 17 Veni Vidi Vici - The tale of the Cotton leaf curl Burrewala virus in India Prem Rajagopalan1, Prashanth Katturi1, Amruta S. Naik1, Meera Kurulekar1, Ravi Kankanallu2, Radha Anandalakshmi1 1 2 Plant-Virus Interactions Lab, Mahyco Research Center, Post Box no. 76, Jalna - 431203, India, Vegetable Research Center, Mahyco, Bengaluru, India 18 Molecular characterization of an isolate of Bean golden mosaic virus infecting soybean in Argentina. P.E Rodríguez-Pardina1, B. Márquez-Martín2, I.G. Laguna1 and J. Navas-Castillo2 1 INTA-IFFIVE, Camino 60 Cuadras Km 5 ½, X5020ICA Córdoba, Argentina. 2Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), 29750 AlgarroboCosta, Málaga, Spain. 19 Tomato severe rugose virus and Tomato mild mosaic virus detected in weeds in natural infections in Southeastern Brazil F.N. Silva, C.S. Rocha, P. Alfenas-Zerbini, G.P. Castillo-Urquiza, A.T.M. Lima & F. M. Zerbini Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil 20 Isolation and characterization of a pepper-infecting curtovirus from Mexico M. Taja, J.A. Mauricio-Castillo, B. Bañuelos-Hernandez and G.R. Argüello-Astorga Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P. 78216 22 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION 21 Identification of a Begomovirus weed (Allosidastrum cf pyramidatum (Lav.) Kropov) associated with the tomato crop in Colombia J.C. Vaca-Vaca and K. Lopez-Lopez Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia. Palmira, Valle del Cauca, Colombia 22 First Identification of a Begomovirus infecting yellow passion fruit in Colombia J.C. Vaca-Vaca, J.F. Betancur-Perez, and K. Lopez-Lopez Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia. Palmira, Valle del Cauca, Colombia 23 Divergent monopartitite geminiviruses: Eragrostis curvula streak virus and Turnip curly top virus Arvind Varsani1,2, Dionne N Shepherd3, Kyle Dent 2,3, Aderito L Monjane3, Edward P Rybicki 3,4, Jahangir Heydarnejad5, Fakhrosadat Khosrowfar6, Hossain Massumi5, Rob W. Briddon7, Darren P Martin4 1 School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand; 2Electron Microscope Unit, University of Cape Town, Rondebosch, Cape Town, , South Africa; 3Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, South Africa; 4Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, Cape Town, South Africa; 5Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran; 6Plant Protection Management, Fars Province, Shiraz, Iran; 7National Institute for Biotechnology and Genetic Engineering, Jhang Road, P.O. Box 577, Faisalabad, Pakistan 24 Characterization of a new begomovirus infecting Solanum lycopersicum in Venezuela K.A Zambranoa, J. Carrillo-Trippb, O. Carballoa, R.F. Rivera-Bustamanteb, and E. Marysa a Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Panamericana Km 11, Caracas, Venezuela. b Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6, Libramiento Norte, P.O. Box 629, C.P. 36500,Irapuato, Guanajuato, México Virus-Host Interactions 25 Four suppressors of RNA silencing are encoded by cotton leaf curl Multan begomovirus complex 6th International Geminivirus Symposium 23 Exit POSTER SESSION Imran Amin1*, Khadim Hussain1,2*, Rashid Akbergenov2, Jitender S. Yadav3, Javaria Qazi1, Shahid Mansoor1, Thomas Hohn2, Claude M. Fauquet3 and Rob W. Briddon1 1 National Institute for Biotechnology and Genetic Engineering, Jhang Road, P.O. Box 577, Faisalabad, Pakistan, 2Institute of Botany, University of Basel, Switzerland, 3ILTAB/Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, USA 26 A reverse genetics approach to discover tomato genes underlying resistance to Tomato yellow leaf curl virus. Eybishtz Assaf, Sade Dagan and Czosnek Hanokh (Henryk) Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel 27 Interaction of the movement protein with a begomovirus-induced cell wall protein. K.M. Balmant1,2, M.C.T. Leite2, C.M. Carvalho1,3, and E.P.B. Fontes1,2 1 INCT em Interações Planta-Praga/Fapemig/CNPq/MCT, 2Departamento de Bioquímica e Biologia Molecular/BIOAGRO, 3Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil, 36570-000 28 Identification of cellular targets for NIG (NSP-interacting GTPase) involved in nucleocytoplasmic transport of proteins. J.P.B. Machado1,2, I.P. Calil1,2, C.M. Carvalho2,3, and E.P.B. Fontes1, 2 1 Dep. Bioquímica e Biologia Molecular/BIOAGRO, 2INCT em Interações Planta-Praga /CNPq/Fapemig/MCT, 3Dep. Fitopatologia/BIOAGRO. Universidade Federal de Viçosa 29 APC7, a component of the Anaphase Promoting Complex, in begomovirus replication F.P. Bruckner1, F.N. Silva2, T.B. Carvalho3, P.C.G. Ferreira3, F.M. Zerbini2, E.P.B. Fontes4 and P. Alfenas-Zerbini1 1 Dep. de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil; 2Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa; 3Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro, RJ, Brazil; 4Dep. de Bioquímica e Biologia Molecular/BIOAGRO, Universidade Federal de Viçosa 30 Differentially expressed proteins in begomovirus-resistant tomato inoculated with Tomato chlorotic mottle virus L.S.T. Carmo1,2, C. Lacorte1, R.S. Fontenele1,2, R.O. Resende2, L.P. Silva1, C. Bloch1, S.G. Ribeiro1, A. Mehta1 24 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION 1 2 Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, Brasil, Universidade de Brasília, Departamento de Biologia Celular, Brasília, Brasil 31 Soybean golden mosaic virus: a new soybean-infecting begomovirus in Southeast Brazil D. Côco1,2, F.M. Zerbini1,3, P. Alfenas-Zernini4, A. Nagata1,5, and E.P.B. Fontes1,2 1 INCT em Interações Planta-Praga, Fapemig/CNPq/MCT, 2Departamento de Bioquímica e Biologia Molecular/BIOAGRO, 3Departamento Microbiologia/BIOAGRO, 4Dep. Microbiologia /BIOAGRO, Univ. Federal de Viçosa, Viçosa, MG, Brasil, 36570-000, 5Embrapa Soja, Londrina, Paraná, Brasil 32 A broad approach to identify cellular proteins required for Geminivirus infection using virus induced gene silencing. T. Rosas-Díaz, R. Lozano-Durán, and E.R. Bejarano. Instituto de Hortofruticultura Subtroplical y Mediterránea “La Superior de Investigación Científicas (IHSM-UMA-SCIC). Mayora”, Univ. de Málaga-Consejo 33 Systemic acquired resistance (SAR) is involved in the geminivirus resistance observed in an accession of Capsicum chinense Jacq. M. A. García-Neria and R. F. Rivera-Bustamante Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P. 36500, Irapuato, Guanajuato, México 34 Quantitative trait loci analysis and whole genome sequences as tools to decipher the resistance to Cabbage leaf curl virus in the Pla-1 ecotype of Arabidopsis thaliana. O. Guerra-Peraza and D. Robertson North Carolina State University, Raleigh NC 27695, USA 35 Analysis of DNA and small RNA accumulation during the interaction of pepper plants with different strains and chimeric viruses of Pepper golden mosaic virus Cecilia Hernandez-Zepeda, Ali M. Idris, Judith K. Brown School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ. USA 36 BCTV and CaLCuV display a different interaction with the cell cycle machinery in Arabidopsis. J.T. Ascencio-Ibañez, L. Sardo, D. Vu, R. Coppersmith, G.P. Accotto and L.K. HanleyBowdoin 6th International Geminivirus Symposium 25 Exit POSTER SESSION Molecular and Structural Biochemistry Department, 128 Polk Hall, North Carolina State University, Raleigh NC 27606 37 The contribution of an alphasatellite-like molecule to symptom severity when in the presence of a begomovirus-beta satellite complex infecting tomato in Oman A.M. Idris1,2, Cecilia Hernández-Zepeda1, J.-K. Zhu2, Judith K. Brown1 1 School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ. USA; 2Plant Stress Genomics and Technology Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia 38 Characterization of recovery phenomenon in Tomato leaf curl New Delhi virus: Correlation between viral DNA, transcripts and miRNA levels Jyothsna. P and Malathi V.G. Advanced Centre for Plant Virology, Div, of Plant Pathology, Indian Agricultural Research Institute, New Delhi, INDIA 39 Molecular dissection of Ty-5, a Tomato yellow leaf curl virus resistance locus in the tomato line TY172 derived from Solanum peruvianum I. Levin, U. Karniel, I. Anbinder, M. Reuveni, S. Nahon, H. Shlomo, L. Chen, and M. Lapidot Institute of Plant Sciences, Agricultural Research Organization, the Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel 40 The rpL10 family as NIK substrates K.V.G. Lopes1,2, A.R. Pataro1,2, F.A. Costa1,2, A.A. Santos1,2, E.P.B. Fontes1,2 1 Departamento de Bioquímica e Biologia Molecular/BIOAGRO, 2 INCT em Interações PlantaPraga/CNPq/Fapemig/MCT, Universidade Federal de Viçosa, Viçosa, MG, Brasil, 36570-000 41 Geminiviruses subvert ubiquitination and inhibit jasmonate signalling by altering CSN-mediated de-rubylation of SCF E3 ligase complexes. R. Lozano-Durán1, T. Rosas-Díaz1, G. Gusmaroli2,4, A.P. Luna1, Renau JP3, X.W. Deng2 and E.R. Bejarano1* (1) Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de MálagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Dept. Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain. (2) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven. Connecticut 06520-8104, USA. (3)UMR INRA 1165 - CNRS 8114 - UEVE, 2 rue Gaston Crémieux, 26 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION CP 5708, 91057 Evry, France. (4)Current Department of Science and Mathematics, University of South Carolina Beaufort, Bluffton, SC 29909 USA 42 Functional diversity of gene silencing suppressors from Tomato yellow leaf curl disease viruses. A.P. Luna1, G. Morilla2, O. Voinnet2 and E.R. Bejarano1* (1) Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Dept. Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain. (2) Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 67084 Strasbourg Cedex, France 43 Genetic silencing of cytoplasmic thioredoxins based on VIGS vector in pepper plants (Capsicum annuum L (cv Anaheim) infected with PepGMV. Luna-Rivero Marianne1, Villanueva-Alonzo Hernan1, Cecilia Hernández-Zepeda2 y Moreno-Valenzuela Oscar A.1 1 Unidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán. Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, México. 2School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA 44 Development of vectors-mediated expression of dsRNA for PTGS activation in Lycopersicum esculentum” D. Medina-Hernandez1, F. Tenllado-Perlalo2, CE. Angulo-Valadez1, and R.J. Holguin-Peña1 1 Biología Molecular de Plantas, CIBNOR, Mar Bermejo No. 195, Col. Playa Palo de Santa Rita Apdo. Postal 128; La Paz, BCS 23090, México. 2Biología Medioambiental, CIB; Ramiro de Maeztu 9, 28040 Madrid. 45 Silencing CchGLP gene increase symptoms severity by geminivirus infections in Capsicum chinense BG-3821 L. Mejia-Teniente1, I. Torres-Pacheco1, C.I. Munoz-Sanchez2, L. Guevara-Olvera2, G. Acosta-García2, M.M. González-Chavira3 R. F. Rivera-Bustamante4, and R.G. GonzalezGuevara1 1 Cerro Universitario Cerro de las Campanas, s/n, Col. Las Campanas. C.P. 76010. Santiago de Querétaro, Querétaro, México. 2Depto. Ingeniería Bioquímica. Instituto Tecnológico de Celaya. Celaya, Gto, México. 3Unidad de Biotecnología del Bajío, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias-Campo Experimental Bajío. Celaya, Gto, México. 4CINVESTAVIrapuato, Carretera Irapuato-León Km 9.6; Irapuato, Gto, México. 6th International Geminivirus Symposium 27 Exit POSTER SESSION 46 Deep sequencing analysis of virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus L. Miozzi, V. Pantaleo, E. Noris, J. Burgyan, G.P. Accotto Istituto di Virologia Vegetale – C.N.R., Strada delle Cacce 73, 10135 Torino, Italy 47 Tomato leaf curl virus or Tomato leaf curl New Delhi virus DNA A and Cotton leaf curl disease betasatellite can cause mild transient symptoms in cotton. Muhammad Saeed National Institute for Biotechnology and Genetic Engineering, PO Box 577, Jhang Road, Faisalabad, Pakistan 48 Altered Gene Expression Profile of Resistant Chilli Genotype Infected With Chilli Leaf Curl Virus Nirbhay Kushwaha1, Pranab Sahu2, Manoj Prasad2 and Supriya Chakraborty1 1 Molecular Virology laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi – 110 067, 2National Institute of Plant Genome Research, New Delhi – 110067 49 Tomato cultivar tolerant to Tomato leaf curl New Delhi virus infection induces virusspecific siRNA accumulation and defense associated host gene expression. P. P. Sahu1*, S. Chakraborty2, M. Singh3, D. Chattopadhyay1, and M. Prasad1* 1 National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi- 110067, INDIA, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, INDIA, 3Indian Institute of Vegetable Research, Gandhinagar, Varanasi-221005, INDIA 2 50 Natural resistance against Mungbean yellow mosaic India virus correlates with early viral transcript degradation and differential siRNA production in Glycine max. Rajiv Kumar Yadav and Debasis Chattpoadhyay Lab 103, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India 51 Global transcriptome profile of Arabidopsis thaliana infected with South African cassava mosaic virus M.E.C. Rey, E. Pierce and F. van Schalk School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, South Africa, 2050 28 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION 52 Transcriptomic and proteomic analysis of Maize infected with Maize streak virus. Rizwan Ali Syed1,3, Zac Mcdonald2,3, Dionne N. Shepherd1, Darren P. Martin2, Suhail Rafudeen1, Edward P. Rybicki1 1 Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, South Africa, 2Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, Cape Town, South Africa, 3 These authors are co-first authors 53 The Tomato yellow leaf curl virus, Sardinian isolate (TYLCSV) C4 protein is a putative transcriptional gene silencing (TGS) suppressor. E.A. Rodriguez-Negrete, R. Lozano-Duran, A.G. Castillo, and E.R. Bejarano Departamento de Genética. Facultad de Biología. Campus de Teatinos. IHSM, UMA-CSIC. 29010 Málaga, Spain 54 Interaction between Geminivirus Replication Protein and the SUMO Conjugating Enzyme Is Required for Viral Infection. Miguel A. Sánchez-Durán1, Mary B. Dallas2, José T. Ascencio-ibañez2, Javier Ruiz-Albert1, Linda Hanley-Bowdoin2 and Eduardo R. Bejarano1 55 The infective cycle of Cabbage leaf curl virus (CaLCuV) is affected by CRUMPLED LEAF (CRL) gene in Arabidopsis thaliana. D.L. Trejo-Saavedra, J.P. Vielle-Calzada and R.F. Rivera-Bustamante Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P. 36500, Irapuato, Guanajuato, México. 56 Arabidopsis synaptotagmins: their roles in virus cell-to-cell movement and plant development. Asako Uchiyama, Harumi Shimada-Beltran, Sondra G. Lazarowitz Cornell University, Dept. of Plant Pathology and Plant-Microbe Biology, Ithaca, NY 14853. USA 57 Gene expression profile of Nicotiana benthamiana infected with South African cassava mosaic virus using a cross-species cDNA microarray approach. F. Van Schalk and M.E.C. Rey School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, South Africa, 2050 6th International Geminivirus Symposium 29 Exit POSTER SESSION 58 Virus-induced gene silencing (VIGS) in plants of commercial and scientific interest. Villanueva-Alonzo, H.1, Guerra-Peraza, O.2, Robertson, D.2, López-Ochoa, L.1, and Moreno-Valenzuela O1 1 Unidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán. Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, México, 2North Carolina State University, Raleigh, NC 27695 59 Functional analysis of the NIK-mediated antiviral signaling pathway in tomato plants. A.A. Santos1, J.A. Condori-Apfata2, O.B. Brustolini2, F.M. Zerbini, 3 E.P.B. Fontes2 1 INCT em Interações Planta-Praga/Fapemig/CNPq/MCT, 2Departamento de Bioquímica e Biologia, Molecular/BIOAGRO, 3Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil, 36570-000 60 Downstream components of NIK-mediated antiviral signaling. C. Zorzatto, J.P.B. Machado, K.V.G. Lopes, A.A. Santos and E.P.B. Fontes INCT em Interações Planta-Praga/CNPq/Fapemig/MCT, Departamento de Bioquímica e Biologia Molecular, BIOAGRO, Universidade Federal de Viçosa, 36570.000 Viçosa, MG, Brazil Epidemiology/Transmission 61 Distribution of Begomovirus associated with tomatoes in the Sultanate of Oman. A.J. Khan, A.O. AL-Matrushi and A. Al-Shihi. Department of Crop Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos University, P.O. Box-34, Al-Khod 123, Sultanate of Oman 62 Distribution and molecular variability of begomovirus affecting the main crops in Cuba: Epidemiological elements. Y. Martínez-Zubiaur; M. Quiñonez, E. Fiallo-Olive, Y. Marrero, M. de los A. Martínez, I. Palenzuela, I. Miranda Departamento de Fitopatología, Centro Nacional de Sanidad Agropecuaria. Apdo 10, San José de las Lajas. Habana, Cuba 63 Multiplex PCR for the simultaneous detection of DNA (geminivirus) and RNA (potyvirus) viruses infecting cassava. Maruthi MN1, Abarshi MM1, Mohammed IU1, Kumar L2, and Legg JP2. 30 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION 1 Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, United Kingdom, 2Internatioanl Institute for Tropical Agriculture (IITA), Ibadan, Nigeria 64 Studies on single and double infection of bipartite begomoviruses by whitefly transmission. M.A. Macedo1,2, V.N. Guimarães1,2, T.G. Pereira1,2, P.P.F. Lemos1,2, A.K. Inoue-Nagata1,2. 1 Universidade de Brasília, 70910-900 Brasília, Brazil; 2Embrapa Vegetables, Brasília/Anápolis, BR 060 Km 09, C.P. 218, 70359-970, Brasília, Brazil 65 Epidemiology and eco-friendly management of yellow mosaic virus of grain legumes in India. YPS Rathi B-55, Pallavpuram - I, Meerut - Meerut - 250110, UP, India. 66 Infectivity of blackgram isolate of Mungbean yellow mosaic India virus on cowpea. Rouhibakhsh1, A. and V. G. Malathi2 Department of Horticulture, Agricultural Faculty, Ilam University, Ilam, Iran. Advance Center for Plant Virology, Division of Plant Pathology, IARI, New Delhi, 110012, India. 67 Study of weeds as begomovirus source to tomato plants in Brazil. S.S. Barreto1, M.A. Reis2, P. P. F. Lemos1 and A. K. Inoue-Nagata1,2 1 Universidade de Brasília, 70910-900 Brasília, Brazil; 2Embrapa Vegetables, BR 060 Km 09, C.P. 218, 70359-970, Brasília, Brazil 68 Molecular characteristics of Cotton leaf curl Multan virus and its associated satellite DNA infecting Hibiscus rosa-sinensis and okra in China. Zifu He1, 2, Dong Di1, Mingjie Mao1, Xiao-man She1, Judith K Brown2 1 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, 510640, China; 2 School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA. Replication/Gene Expression 69 Two strains of Euphorbia mosaic virus are incompatible in replication and contain short Rep sequences in the BV1 promoter region. B. Bañuelos-Hernández, J. Gregorio-Jorge, G.R. Argüello-Astorga 6th International Geminivirus Symposium 31 Exit POSTER SESSION Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P. 78216 70 The impact of geminiviral proteins on the nuclear architecture Krenz B. 1, Neugart F. 2, Jeske H. 1 and Kleinow T1 1 University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany , 2University of Stuttgart, Institute of Cell Biology, Stuttgart, Germany 71 Visualization of Abutilon mosaic virus replication-associated self-interaction by bimolecular fluorescence complementation in planta protein Krenz B. 1, Neugart F. 2, Kleinow T. 1 and Jeske H. 1 1 University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany , 2University of Stuttgart, Institute of Cell Biology, Stuttgart, Germany 72 The begomoviral conserved late element (CLE) is a functional target of plant transcription factors M. Cantú-Iris1, A. Juárez-Reyes1, R. Rivera-Bustamante2, and G.R. Argüello-Astorga1 1 Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P. 78216. 2 Departamento de Ingeniería Genética, Cinvestav Irapuato, P.O. Box 629, C.P. 36500, Irapuato, Guanajuato, México. E-mail: [email protected] 73 Analysis of trans-replication of a betasatellite by a begomovirus Khadim Hussain, Mazhar Hussain, Shahid Mansoor and Rob W. Briddon National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577. Faisalabad Pakistan 74 Characterization of strong promoters from American begomoviruses A. Londoño, L.R. Riego-Ruiz, G.R. Arguello-Astorga División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica. Camino a la Presa San José 2055, Col. Lomas 4 sección, P.O. Box 78216, San Luis Potosí, SLP, México 32 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION Genetic Diversity/Evolution 75 Genetic diversity by extensive recombination in the complex of monopartite begomoviruses infecting sweet potato in Brazil L.C. Albuquerque1,2,4, B. Pinheiro1,2, S.G. Ribeiro3, R.O. Resende2, E. Moriones4, A.K. Inoue-Nagata1,2, and J. Navas-Castillo4 1 Embrapa Vegetables, C.P. 218, Brasília, DF, 70.359-970, Brazil. 2Universidade de Brasília, Brasília, DF, 70910-970, Brazil. 3Embrapa Cenargen, C.P. 02372, Brasília, DF, CEP 70970900, Brazil. 4Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSMUMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain. 76 The first monopartite begomoviruses discovered in Western Hemisphere endemic eudicots: evidence for swepovirus diversification in ‘wild’ and ‘cultivated’ hosts J. K. Brown1, H. Delatte2, J. Bird,3 and Ali M. Idris1,4 1 School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ, USA; 2CIRAD, UMR PVBMT, Pôle de Protection des Plantes, 7 chemin de l’IRAT, 97410 Saint Pierre, La Réunion, France; 3College of Agricultural Sciences, University of Puerto Rico, Rio Piedras, PR 00928; 4Plant Stress Genomics and Technology Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia 77 Genetic structure of tomato-infecting begomovirus populations in two growing regions of Southeastern Brazil G.P. Castillo-Urquiza1, P. Alfenas-Zerbini1, J.E.A. Beserra-Junior1, E.S.G. Mizubuti1, A. Varsani2, D.P. Martin3 and F.M. Zerbini1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil; School of Biological Sciences, University of Canterbury, Private bag, Christchurch, 8140, New Zealand; 3Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa 2 78 Epidemiology and molecular characterization of Tomato curly stunt virus and its insect vector Bemisia tabaci in South Africa L.L. Esterhuizen1, S.W. Van Heerden2, M.E.C. Rey3 and H. Van Heerden4 1 Department of Biochemistry, University of Johannesburg, Johannesburg, South Africa, 2Sakata Vegenetics RSA (Pty) Ltd., Lanseria, South Africa, 3Department of Cell and Molecular Biochemistry, University of Witwatersrand, Johannesburg, South Africa, 4Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa 6th International Geminivirus Symposium 33 Exit POSTER SESSION 79 Bipartite and monopartite begomoviruses infecting Ipomoea spp. and Merremia spp. in Venezuela E. Fiallo-Olivé1,2, B. Márquez-Martín1, F. Geraud-Pouey3, D. T. Chirinos3, J. NavasCastillo1 and E. Moriones1 1 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de MálagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain. 2Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 3 Universidad del Zulia, Maracaibo, Zulia , Venezuela. 80 Begomovirus diversity and recombination in the Caribbean, a hot ‘melting pot’ in the New World. E. Fiallo-Olivé1,2, E. Moriones2, J. Navas-Castillo2 and Y. Martínez-Zubiaur1 1 Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 2Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, 29750 Algarrobo-Costa, Málaga, Spain 81 Diversity and evolutionary dynamics of begomovirus infecting a wild crop relative, Capsicum annuum var. glabriusculum P. González-Jara1, M. Rodelo-Urrego1, A. Fraile1, D. Piñero2 and F. García-Arenal1 1 Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Universidad Politécnica de Madrid. Campus de Montegancedo, 29223 Pozuelo de Alarcón, Spain. 2Instituto de Ecología, Universidad Nacional Autónoma de México, AP 70-275, México, D.F, Mexico. 82 Molecular characterization of Sweet potato leaf curl virus (SPLCV) Korea isolates Jungan Park1, Hyejung Lee1, Yuchul Jung1, Jaelim Yu1, Hong-Soo Choi2, and Sukchan Lee1 1 Department of Genetic Engineering, Sungkyunkwan University, Korea, 2Agricultural Microbiology Division, National Academy of Agricultural Science, Korea 83 Agroinoculation of cloned Honeysuckle yellow vein virus (HYVV) isolated from Lonicera japonica and its recombination study Hyejung Lee, Gunsup Lee, Jungan Park, Seungchan Cho, Jisook Chae, Eunbyul Ji, Kangsan Roh, Taejune Jun, Sukchan Lee Department of Genetic Engineering, Sungkyunkwan University, Korea 34 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION 84 Investigations towards understanding the evolution and global movement of Abutilon mosaic virus Simona Kraberger1, Daisy Stainton1, Karyna Rosario2, Holger Jeske3, Darren P Martin4, David Collings1 and Arvind Varsani1, 5 1 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; 2College of Marine Science, University of South Florida, St Petersburg, Florida; 3Department of Molecular Biology and Plant Virology, Institute of Biology, University of Stuttgart, Stuttgart, Germany; 4 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; 5Electron Microscope Unit, University of Cape Town, Cape Town, South Africa 85 Discovering circomics! Deep insights into plant viral circular genomes Björn Krenz1, Tobias Paprotka2, Judith Horn1, Benjamin Schäfer1, Patricia Wyant1, Stephan Strohmeier1 and Holger Jeske1 1 University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany , 2Viral Mutation Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA. 86 Phylogenetic analysis of TbLCV and its evidence for recombination with TYLCV Hyejung Lee1, Jungan Park1, Eui-Joon Kil¹,Mangyong Jung1,Jin-gweonYang¹, Hong-Soo Choi2, and Sukchan Lee1* 1 Department of Genetic Engineering, Sungkyunkwan University, Korea, 2Agricultural Microbiology Division, National Academy of Agricultural Science, Korea 87 Phylogeny and recombination analysis of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea Hyejung Lee¹, Jungan Park¹, Eui-Joon Kil¹, Jin-gweon Yang¹, Kyeong-yeoll Lee², Hong-Soo Choi³, Sukchan Lee¹ ¹Department of Genetic Engineering, Sungkyunkwan University, Korea, 2School of Applied Biosciences, Kyungpook National University, Korea, ³Agricultural Microbiology Division, National Academy of Agricultural Science, Korea 88 Diversity of tomato begomoviruses in the three major tomato growing states of Brazil A.T.M. Lima1, F.Y.B. Naito2, F.N. Silva1, C.S. Rocha1, D.R. Barros1, G.P.C. Urquiza1, A.W.O. Malta1, M.F. Basso1, M.T. Godinho1, E.S.G. Mizubuti1, F.M. Zerbini1 and A.K. Inoue-Nagata2,3 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil; Dep. de Fitopatologia/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, 70910-900, Brazil; 3Embrapa Hortaliças, BR 060 Km 09, C.P. 218, Brasília, DF, 70359-970, Brazil 2 6th International Geminivirus Symposium 35 Exit POSTER SESSION 89 Begomoviruses infecting weeds in Bolivia A.T.M. Lima1, F.N. Silva1, C.S. Rocha1, T.F.S. Antunes1, D.R. Barros1, G.P. Rosales2, N. Ortuño2, A. Gandarillas2, R.O. Resende3 and F. M. Zerbini1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil; Fundación PROINPA, Av. Meneces s/n. Km. 4, Zona El Paso, Cochabamba, Bolivia; 3Dep. de Biologia Celular/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, 70910-900, Brazil. 2 90 Genetic analysis and phylogeographic history of MSV reveals the spatial and temporal origins of contemporary MSV-A recombinant lineages and identifies southern Africa as the ultimate origin of Maize streak disease A. L. Monjane, G. W. Harkins, D. P. Martin, P. Lemey, D. N. Shepherd, P. Lefeuvre, E. P. Rybicki , B. E. Owor , R.S. Ali, B. Flett, M. Ramusi, S. Oluwafemi, and A. Varsani Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa 91 Diversity of Dicot-infecting Mastreviruses Huma Mumtaz1, Safaa G. Kumari2, Nazia Nahid1, Darren P. Martin3, Shahid Mansoor1 and Rob W.Briddon1 1 Agriculture Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan, 2Virology Laboratory, International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria, 3Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa 92 Genetic diversity of DNA satellites associated with Ipomoea-infecting monopartite begomoviruses H.P. Trenado1, E. Fiallo-Olivé1,2, G. Lozano1, D. Chirinos3, F. Geraud-Pouey3, E. Moriones1, R.W. Briddon4, and J. Navas-Castillo1 1 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de MálagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29750 Algarrobo-Costa, Málaga, Spain. 2Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 3 Universidad del Zulia, Maracaibo, Zulia, Venezuela. 4Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan 36 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION 93 Begomovirus species diversity in South American weeds T.G. Pereira1,2, F.Y.B. Naito1,2, R.S. Fontenele1,3, R. Blawid1,3, F.R. Fernandes4, A.T.M. Lima4, F.N. Silva4, C.S. Rocha4, G.P. Rosales5, C.L. Oliveira1,2, R.O. Resende1, F.M. Zerbini4, S.G. Ribeiro3, F. J. L. Aragão3, A.K. Inoue-Nagata1, 2 1 Universidade de Brasília,CEP 70910-900 Brasília, Brazil. 2Embrapa Vegetables, BR 060 Km 09, C.P. 218, CEP 70359-970, Brasília, Brazil. 3Embrapa Recursos Genéticos e Biotecnologia, Pq Estação Biológica, Brasília, Brazil. 4Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil. 5Fundación PROINPA, Av. Meneces s/n. Km. 4, Zona El Paso, Cochabamba, Bolivia 94 Begomovirus occurring on tomatoes in Brazil and their diversity in Goiás state B. Pinheiro1,2, L. C. Albuquerque1,2, D. P. Martin3, A. Varsani3, and Inoue-Nagata A.K.1,2 1 Universidade de Brasília, 70910-900, Brasília, Brazil. 2Embrapa Vegetables, BR 060 Km 09, C.P. 218, 70359-970, Brasília, Brazil. 3University of Cape Town, Rondebosch, Cape Town, 7701, South Africa 95 Genetic structure of a population of the begomovirus Bean golden mosaic virus (BGMV) that infects lima bean (Phaseolus lunatus L.) in the state of Alagoas, Brazil R. Ramos-Sobrinho1,2, S.J.C. Silva1,3, T.A.L. Silva4, S.G. Ribeiro4, G.S.A. Lima2, I.P. Assunção2 and F.M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, UFV, Viçosa, MG, 36570-000, Brazil; 2Dep. de Fitopatologia, CECA, UFAL, Maceió, AL, 57072-970, Brazil; 3Dep. de Fitopatologia, UFRPE, Recife, PE, 52171900, Brazil; 4Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-917, Brazil 96 Recombination and pseudorecombination driving the evolution of Tomato severe rugose virus and Tomato rugose mosaic virus: Two distinct DNA-As sharing the same DNA-B. F.N. Silva1, A.T.M. Lima1, C.S. Rocha1, M. Alves-Júnior, M.1 Hallwass2, A.K. InoueNagata3 and F. M. Zerbini1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil; Dep. de Biologia Celular/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, 70910-900, Brazil; 3Embrapa Hortaliças, BR 060 Km 09, C.P. 218, Brasília, DF, 70359-970, Brazil 2 97 Low diversity of Begomovirus species infecting Cleome affinis in Northeastern Brazil S.J.C. Silva1,2, G.P. Castillo-Urquiza1, G. Pio-Ribeiro2, I.P. Assunção3, G.S.A. Lima3, F.M. Zerbini1 6th International Geminivirus Symposium 37 Exit POSTER SESSION 1 Dep. de Fitopatologia/BIOAGRO, UFV, Viçosa, MG, 36570-000, Brazil; 2Dep. de Fitopatologia, UFRPE, Recife, PE, 52171-900, Brazil; 3Dep. de Fitopatologia, CECA, UFAL, Maceió, AL, 57072970, Brazil. 98 High diversity of Begomovirus species infecting Macroptilium spp. in Northeastern Brazil S.J.C. Silva1,2, G.P. Castillo-Urquiza1, G. Pio-Ribeiro2, I.P. Assunção3, G.S.A. Lima3, F.M. Zerbini1 1 Dep. de Fitopatologia/BIOAGRO, UFV, Viçosa, MG, 36570-000, Brazil; 2Dep. de Fitopatologia, UFRPE, Recife, PE, 52171-900, Brazil; 3Dep. de Fitopatologia, CECA, UFAL, Maceió, AL, 57072970, Brazil 99 The perspective for the use of genetically modified common beans in Brazil based on genetic diversity of Bean golden mosaic virus T. A. L. Silva, R. S. Fontenele, J. C. Faria, F. J. L. Aragão, and S. G. Ribeiro Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica – Brasília, DF, Brazil 100 Genetic Diversity of Banana bunchy top virus (BBTV) in the South Pacific Tongan Archipelago. Daisy Stainton1, Simona Kraberger1, Karyna Rosario2, Milen Marinov1, Elizabeth Wiltshire1, Matthew Walters1, Samieluela Lolohea3, Mana’ia Halafihi4, Ika Katoa4, Waikato Aholelei 4 Luseane Taufa4, David Collings1, Darren P Martin5and Arvind Varsani1, 6. 1 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; 2College of Marine Science, University of South Florida, St Petersburg, Florida; 3Tonga College, Tonga’tapu, Kingdom of Tonga; 4Ministry of Agriculture and Food, Forests and Fisheries, Kingdom of Tonga; 5 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; 6Electron Microscope Unit, University of Cape Town, Cape Town, South Africa 101 Genetic analysis and phylogeographic history of MSV reveals the spatial and temporal origins of contemporary MSV-A recombinant lineages and identifies southern Africa as the ultimate origin of Maize streak disease A. L. Monjane, G. W. Harkins, D. P. Martin, P. Lemey, D. N. Shepherd, P. Lefeuvre, E. P. Rybicki , B. E. Owor , R.S. Ali, B. Flett, M. Ramusi, S. Oluwafemi, and A. Varsani Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa 38 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION Gene Expression 102 The V2 protein of Monopartite begomovirus determines virulence, hypersensitive response and suppression of posttranscriptional gene silencing. Pradeep Sharma, and Masato Ikegami* Division of Crop Improvement, Directorate of Wheat Research, Karnal 132 001, India *Graduate School of Agricultural Sciences, Tohoku University, Sendai 981-8555, Japan Virus induced gene silencing 103 Silencing of PPR gene in garlic (Allium sativum L) “blanco perla” resistant to white rot using V-VIGS derived of PHYVV. L. Guevara-Olvera, G. Acosta-García, F. Delgadillo-Sanchez, R. F. Rivera-Bustamante and L. Chairez-Rodríguez. Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Av. Tecnológico y A. García Cubas s/n, C.P. 38020, Celaya, Guanajuato, México. Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P. 36500, Irapuato, Guanajuato, México. INIFAP, Campo experimental Bajío, km 6 Carr. Celaya-San Miguel de Allende, Apdo. Postal 112, Celaya, Guanajuato CP 38000 104 Development of an efficient system for assessing gene function in the cotton plant using virus-induced gene silencing (VIGS) Cecilia Hernandez-Zepeda, ZiFu He, Judith K. Brown School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ. USA Management/Control 105 RNAi-resistance to bipartite Begomoviruses in genetically modified tomatoes L.M. Almeida, T.M. Cipriano, K. Bonfim, E.O.P.L. Nogueira, S. G. Ribeiro, F. J.F.L. Aragão Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, 70770-900, Brasília, DF, Brasil 6th International Geminivirus Symposium 39 Exit POSTER SESSION 106 Broad-spectrum resistance to Brazilian bipartite Begomovirus species in tomato lines carrying the Ty-1 or tcm-1 loci R. Blawid1,2, R.F. Fontenele1, C. Lacorte1, R.C.P. Carvalho2, R.O. Resende2, L.S. Boiteux3, M.E.N. Fonseca3, A. C. M. Brasileiro1, and S.G. Ribeiro1 1 Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica - Brasília-DF, Brazil; Departamento de Biologia Celular, UNB, Brasília-DF, Brazil; 3Embrapa Hortaliças, Rodovia Brasília/Anápolis BR 060 Km 09, Gama-DF, Brazil 2 107 The biosafety of siRNA transgenic common beans resistant to Bean golden mosaic virus. J. C. Faria1, F. J. L. Aragão2 1 2 Embrapa Arroz e Feijão, Caixa Postal 179, Santo Antônio de Goiás, GO CEP 75375-000 - Brasil; Embrapa Recursos Genéticos e Biotecnologia, Caixa Postal ----, Brasília, DF CEP - Brasil 108 Segregation pattern of genetic resistance to Tomato yellow leaf curl virus Israel (TYLCV-IL) in F2:3 families derived from the tomato hybrid ‘TyKing’ R.C. Pereira-Carvalho1,2,3, L.S. Boiteux1, M.E.N. Fonseca1, J.A. Díaz-Pendón3, E. Moriones3, R. Fernández-Muñoz3, AND R.O. Resende2 1 National Center for Vegetable Crops Research (CNPH), Embrapa Vegetable Crops, Brasilia-DF, Brazil; 2Departments of Plant Pathology and Cell Biology, UnB, Brasilia-DF, Brazil; 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), 29750 AlgarroboCosta, Málaga, Spain 109 Sustainability of Indian agriculture sans pesticides YPS Rathi B-55, Pallavpuram - I, Meerut - Meerut - 250110, UP, India. Mixed Infection 110 Association of two begomoviruses with severe golden mosaic disease of cowpea Naimuddin and Mohammad Akram Division of Crop Protection, Indian Institute of Pulses Research, Kanpur-208 024, India 40 4th International ssDNA Comparative Virology Workshop Exit POSTER SESSION Etiology 111 Is tomato leaf deformation disease caused by the DNA-A component of a novel begomovirus from Peru? B. Márquez-Martín1, L. Aragón-Caballero2, E. Fiallo-Olivé1,3, J. Navas-Castillo1, and E. Moriones1 1 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de MálagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, 29750 Algarrobo-Costa, Málaga, Spain; 2Facultad de Agronomía, Universidad Nacional Agraria La Molina, La Molina, Lima, Perú; 3Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 112 The first infectious clone of a “swepovirus” H.P. Trenado, A.F. Orílio, B. Márquez-Martín, E. Moriones, and J. Navas-Castillo Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de MálagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, 29750 Algarrobo-Costa, Málaga, Spain. Survey 113 Survey on begomoviruses on cucurbit species in Brazil M.F. Lima and A.K. Inoue-Nagata Embrapa Vegetables, BR 060 Km 09, Caixa Postal 218 CEP 70359-970, Brasília, Distrito Federal, Brazil Other 114 Use of Virus-Induced Gene Silencing (VIGS) to accelerate genetic gain. Alvarez-Venegas, R., Zhang, Y., Kraling, K., and Tulsieram, L. Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P. 36500, Irapuato, Guanajuato, México. 115 Analysis of geminivirus literature in the last decade. Favorite journals, most prolific authors, geographic distribution. R. Fajardo, M.A. Garcia-Neria, D.L. Trejo-Saavedra, R.F. Rivera-Bustamante Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P. 36500, Irapuato, Guanajuato, México. 6th International Geminivirus Symposium 41 Exit POSTER SESSION 116 The spread of Tomato yellow leaf curl virus (TYLCV) from the Middle East to the world Pierre Lefeuvre, Gordon Harkins, Darren P. Martin, Philippe Lemey, Alistair J. A. Gray, Sandra Meredith, Francisco Lakay, Dionne N. Shepherd, Hossain Massumi, Jahangir Heydarnejad, and Arvind Varsani 117 Use of rolling circle amplification to enable rapid completion of Koch’s postulates for circular DNA viruses. M. Lapidot1, J.E. Polston2, D. Guenoune-Gelbart1, T. Sufrin-Ringwald1, H. Capobianco2, V. Gaba3 Departments of 1Vegetable Research and 3Plant Pathology, Volcani Center, ARO, P.O.Box 6, Bet Dagan 50250, Israel, 2Department of Plant Pathology, University of Florida, Gainesville, FL 32611, U.S.A. 118 Characterization of a New Virus Discovered by Vector-Enabled Metagenomics on the Whitefly Vector J. E. Polston1, T. F.F. Ng2, V. Campoverde1, H. Capobianco1, T. Xia1, M. Breitbart2 1 Dept of Plant Pathology, University of Florida, Gainesville, FL 32611, and 2 College of Marine Science, University of South Florida, 140 7th Avenue South, Saint Petersburg, Florida, USA 119 Evidence of Begomovirus mixed infections affecting tomato (Solanum lycopersicum) plants in Colombia J. C. Vaca-Vaca, J. F. Betancur-Perez, and K. Lopez-Lopez. Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia. Palmira, Valle del Cauca, Colombia. PARVOVIRIDAE Epidemiology/transmission 120 Human parvovirus 4 (PARV4): lessons from epidemiologic studies performed in south-eastern France. M. Touinssi, P. De Micco, and P. Biagini UMR CNRS 6578 Equipe « Emergence et co-évolution virale », Etablissement Français du Sang Alpes-Méditerranée et Université de la Méditerranée, 27 Bd. Jean Moulin, 13005 Marseille, France. 42 4th International ssDNA Comparative Virology Workshop AUTHOR INDEX Exit AUTHOR INDEX A B Abarshi MM, 30 Accotto, G.P., 8, 25, 28 Acosta-García, G., 27, 39 Akbergenov, R., 24 Akram, M., 40 Albuquerque, L.C., 33, 37 Alfenas-Zerbini, P., 9, 20, 22, 24, 25, 33 Ali M. Idris, 25, 33 Ali, R.S., 36, 38 Alistair J. A. Gray, 42 AL-Matrushi, A.O., 30 Almeida, L.M., 39 Almeida, M. M. S., 13, 21 Al-Shihi, A., 30 Álvarez-Venegas, R., 6, 41 Alves-Júnior, M., 37 Ambriz-Granados, S., 21 Amin, I., 8, 24 Amruta S. Naik, 22 Anandalakshmi, R., 13, 22 Anbinder, I., 26 Angulo-Valadez, CE., 27 Antunes, T.F.S., 20, 36 Aragão, F. J. L., 37, 38, 39, 40 Aragón-Caballero, L., 41 Aranha, SA., 21 Argüello-Astorga, G.R., 20, 21, 22, 31, 32 Asako Uchiyama, 6, 29 Ascencio-Ibañez, JT., 6, 25, 29 Assunção, I.P., 37, 38 Balmant, K.M., 24 Bañuelos, B, 6 Bañuelos-Hernandez, B., 22, 31 Barbosa, J.C., 21 Barreto, S.S., 31 Barros, D.R., 20, 35, 36 Basso, M.F., 35 Bejarano, E.R., 4, 25, 26, 27, 29 Benjamin Schäfer, 35 Bergsten-Torralba, L.R., 19 Beserra-Junior, J.E.A., 33 Betancur-Perez, J. F., 23, 42 Biagini , P., 4, 42 Bird, J., 33 Bisaro, D., 7 Blawid, R., 21, 37, 40 Bloch, C., 24 Boiteux, L.S., 40 Bonfim, K., 39 Borkosky, SS, 19 Bragg, R., 21 Brasileiro, A. C. M., 40 Breitbart, M., 5, 20, 42 Briddon, R., 11, 12, 23, 24, 32, 36 Brown, JK., 11, 12, 21, 25, 26, 31, 33, 39 Bruckner, F.P., 24 Brustolini, O.B., 30 Burgyan, J., 28 C Calil, I.P., 24 Camacho-Beltrán E, 22 Campoverde, V., 42 Cantú-Iris, M., 21, 32 Capobianco, H., 42 6th International Geminivirus Symposium Carballo, O., 23 Cárdenas-Cornejo, Y., 21 Carmo, L.S.T., 24 Carrillo-Tripp, J., 23 Carvalho, C.M., 24 Carvalho, T.B., 24 Castillo, A.G., 29 Castillo-Urquiza, G.P., 22, 33, 38 Chairez-Rodríguez, L., 39 Chakraborty, S., 14, 21, 28 Chattopadhyay, B., 21 Chattopadhyay, D., 28 Chen, L., 26 Chirinos, D. T., 34 Chirinos, D., 36 Cipriano, T.M., 39 Côco, D., 25 Collings, D., 35, 38 Condori-Apfata, J.A., 30 Coppersmith, R., 25 Cortey, M, 20 Costa, F.A., 26 D Dagan, S., 24 Dallas, M.B., 29 De Micco, P., 42 Delatte, H., 33 Delgadillo-Sanchez, F., 39 Deng, X.W., 26 Dent, K., 23 Díaz de Arce, H., 20 Díaz-Pendón, J.A., 40 Diniz-Mendes, L., 15, 19 Dionne N Shepherd, 23, 29, 42 Domínguez, P, 20 Dong Di, 31 Duffy, S., 13 45 Exit AUTHOR INDEX E Esterhuizen, L. L., 14, 33 Eui-Joon Kil, 35 Eunbyul Ji, 34 Eybishtz Assaf, 15, 24 F Fajardo, R., 41 Faria, J. C., 15, 38, 40 Fauquet, CM., 4, 11, 13, 24 Fernandes, F.R., 37 Fernández-Muñoz, R., 40 Ferreira, P.C.G., 24 Fiallo-Olive, E., 13, 30, 34, 36, 41 Flett, B., 36, 38 Fonseca, M.E.N., 40 Fontenele, R. S., 21, 24, 37, 38 Fontenele, R.F., 40 Fontes, E.P.B., 10, 24, 25, 26, 30 Fraile, A., 34 Frías, MT, 20 G G. Ribeiro, S., 21, 38, 39 G.P.C. Urquiza, 35 Gaba, V., 42 Gámez-Jiménez, C, 22 Gandarillas, A., 20, 36 Ganges, L, 20 García-Arenal, F., 34 García-Neria, M. A., 25, 41 Garry Sunter, 9 Geraud-Pouey, F., 34, 36 Godinho, M.T., 35 González-Chavira, M.M., 27 46 Gonzalez-Guevara, R.G., 27 González-Jara, P., 34 Gregorio-Jorge, J., 31 Gronenborn, B, 10 Guenoune-Gelbart, D., 42 Guerra-Peraza, O., 15, 25, 30 Guevara-Olvera, L., 27, 39 Guimarães, V.N., 31 Gusmaroli, G., 26 H Hallwass, M., 37 Hanley-Bowdoin, L, 7, 25, 29 Hanokh C., 24 Harkins, GW., 14, 36, 38, 42 Hausen, HZ., 19 He, Z., 31, 39 Hernández-Zepeda, C, 5, 21, 25, 26, 27, 39 Heydarnejad, J., 23, 42 Hitzeroth, II., 21 Hoffmann, L.V., 21 Hohn, T., 24 Holguin-Peña, R.J., 27 Hong-Soo Choi, 34, 35 Horn, J., 35 Hussain, K., 24, 32 Hussain, M., 32 I Idris, A.M., 26 Ika Katoa, 38 Ikegami, M., 39 Inoue-Nagata, A.K., 21, 31, 33, 35, 37, 41 J Jeske, H., 12, 32, 35 Jian-Wei, F., 19 Jin-gweon Y., 35 Jisook Chae, 34 Juárez-Reyes, A., 32 Jun, T., 34 Jung, Y., 34 Jyothna P, 6, 26 K Kankanallu, R., 22 Karniel, U., 26 Katturi, P., 22 Khan, A.J., 30 Khosrowfar, F., 23 Khushwaha, N., 21 Kimmel, R, 19 Kitajima, E.W., 20 Kleinow T, 32 Kopp-Schneider, A., 19 Kraberger, S., 20, 35, 38 Kraling, K, 41 Krenz, B, 9, 32, 35 Kumar L, 30 Kumar Yadav, R., 28 Kumari, SG., 36 Kurulekar, M., 22 Kushwaha, N., 28 Kyeong-yeoll Lee, 35 L Lacorte, C., 24, 40 Laguna, I.G., 22 Lakay, F., 42 Lapidot, M., 14, 26, 42 Lazarowitz, SG., 7, 29 Lee, G., 34 Lee, H., 34, 35 Lee, S., 34, 35 Lefeuvre, P., 36, 38, 42 Legg JP, 30 4th International ssDNA Comparative Virology Workshop Exit AUTHOR INDEX Leite, J. P. G., 19 Leite, M.C.T., 24 Lemey, P., 36, 38, 42 Lemos, P. P. F., 31 Levin, I., 16, 26 Leyva-López, N.E, 22 Ligia M. Almeida, 16 Lima, A.T.M., 20, 22, 35, 36, 37 Lima, G.S.A., 37, 38 Lima, M.F., 41 Londoño, A., 9, 20, 32 Lopes, K.V.G., 26, 30 Lopez-Lopez, K., 23, 42 López-Ochoa, L, 30 Lozano, G., 36 Lozano-Durán, R., 25, 26, 29 Luna, A.P., 26, 27 Luna-Rivero M, 27 M Macedo, M.A., 31 Machado, J.P.B., 24, 30 Magallanes-Tapia, M.A, 22 Malathi V.G, 26 Malta, A.W.O., 35 Mana’ia Halafihi, 38 Mangyong Jung, 35 Mao, M., 31 Marinov, M, 20, 38 Márquez-Martín, B., 22, 34, 41 Marrero, Y., 30 Martin, D. P., 33, 36, 37, 38 Martin, D., 20 Martin, DP, 12, 23, 29, 35, 36, 38, 42 Martínez, MA., 30 Martínez-Zubiaur, Y., 30, 34 Maruthi MN, 30 Marys, E., 23 Massumi, H., 23, 42 Mauricio-Castillo, J.A., 22 Mcdonald, Z., 29 Medina-Hernandez, D., 27 Mehta, A., 24 Mejia-Teniente, L., 27 Méndez-Lozano J, 22 Meredith, S., 42 Miagostovich, M.P., 19 Miozzi, L., 28 Miranda, I., 30 Mizubuti, E.S.G., 33, 35 Mohammed IU, 30 Monjane, A. L., 14, 23, 36, 38 Moreno-Valenzuela O, 27, 30 Morilla, G., 27 Moriones, E., 15, 33, 34, 36, 40, 41 Mubin, M., 8 Mukherjee, SK., 10 Mumtaz, H., 5, 36 Munoz-Sanchez, C.I., 27 N Nagata, A., 25 Nahon, S., 26 Naimuddin, 40 Naito, F.Y.B., 35, 37 Navas-Castillo, J., 5, 11, 22, 33, 34, 36, 41 Nazia Nahid, 36 Neugart F, 32 Ng, T. F.F., 42 Niel, C., 19 Nogueira, E.O.P.L., 39 Noris, E., 28 Núñez, JI, 20 6th International Geminivirus Symposium O Oliveira, C.L., 37 Oluwafemi, S., 36, 38 Orduño-Vega, W, 22 Orílio, A.F., 41 Ortuño, N., 20, 36 Owor, B. E., 36, 38 P Palenzuela, I., 30 Pantaleo, V., 28 Paprotka, T., 35 Park, J., 34, 35 Pataro, A.R., 26 Paula, V.S. De, 19 Percedo, MI, 20 Pereira, T.G., 14, 31, 37 Pereira-Carvalho, R.C., 15, 40 Perera, CL, 20 Pérez, LJ, 20 Pierce, E., 28 Pinheiro, B., 33, 37 Pinho-Nascimento, C. A., 19 Piñero, D., 34 Pio-Ribeiro, G., 38 Polston, J.E., 13, 42 Pooggin, M., 9 Prasad, M., 28 Prem Rajagopalan, 22 Q Qazi, J., 24 Quiñonez, M., 30 R Rafudeen, S., 29 Ramos-Sobrinho, R., 37 Ramusi, M., 36, 38 Rathi, YPS., 31, 40 Regnard, GL., 21 47 Exit AUTHOR INDEX Reis, M.A., 31 Renau JP, 26 Resende, R.O., 20, 24, 33, 36, 37, 40 Reuveni, M., 26 Rey, M.E.C., 28, 29, 33 Ribeiro, SG., 24, 33, 37, 40 Riego-Ruiz, LR, 20, 32 Rivera-Bustamante, RF., 8, 23, 25, 27, 29, 32, 39, 41 Rizwan Ali Syed, 29 Robertson, D., 6, 25, 30 Rocha, C.S., 20, 22, 35, 36, 37 Rodelo-Urrego, M., 34 Rodriguez-Negrete E.A., 8, 29 Rodríguez-Pardina, P.E., 22 Roh, K., 34 Romero-Romero, J.L, 22 Rosales, G.P., 20, 36, 37 Rosario, K., 5, 20, 35, 38 Rosas-Díaz, T., 25, 26 Ruiz-Albert, J., 29 Rybicki, E. P., 21, 23, 29, 36, 38 S Saeed, M., 28 Sahu, P. P., 28 Samieluela L, 38 Sánchez-Durán, MA., 29 Santos, A.A., 26, 30 Sardo, L., 25 Segalés, J, 20 Seungchan Cho, 34 Shahid Mansoor, 24, 32, 36 Sharma, P., 8, 39 48 Shepherd, D. N., 36, 38 Shimada-Beltran, H., 29 Shlomo, H., 26 Silva, F.N., 20, 22, 24, 35, 36, 37 Silva, L.P., 24 Silva, S.J.C., 37, 38 Silva, T. A. L., 37, 38 Singh, M., 28 Singh, A. K., 21 Stainton, D., 16, 20, 35, 38 Strohmeier, S., 35 Sufrin-Ringwald, T., 42 T Taja, M., 22 Tarradas, J, 20 Taufa, L., 38 Tenllado-Perlalo, F., 27 Tijssen, P., 4 Torres-Pacheco, I., 27 Touinssi, M., 42 Trejo-Saavedra, D.L., 29, 41 Trenado, H.P., 36, 41 Tulsieram, L, 41 Vu, D., 25 W Waikato Aholelei, 38 Walters, M., 38 Wege, C, 7 Whitley, C, 19 Wiltshire, E., 38 Wyant, P., 35 X Xia, T., 42 Xiao-man She, 31 Y Yadav, JS., 24 Yu, J., 34 Z Zambrano, K.A., 23 Zerbini, F.M., 12, 20, 22, 24, 25, 30, 33, 35, 36, 37, 38 Zhang, Y, 41 Zhou, X., 7 Zhu, J.K., 26 Zorzatto, C., 30 V Vaca-Vaca, J. C., 23, 42 Van Heerden, H., 33 Van Heerden, S.W., 33 Van Schalk, F., 28, 29 Varsani, A. 5, 11, 20, 21, 23, 33, 35, 36, 37, 38, 42 Vetten, HJ., 4 Vielle-Calzada, J.P., 29 Villanueva-Alonzo H, 27, 30 Villiers, EM de, 10, 19 Vinothkumar, R., 21 Voinnet, O., 27 4th International ssDNA Comparative Virology Workshop
