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MEET consortium workshops
Dates: Madrid, October 5th – 9th 2015
Venue: Fundación Centro Nacional de Investigaciones Cardiovasculares
Principal Investigator: Jose A. Enríquez
Supervisors: C. Jiménez, R. Acín, A. Guarás e I. Martínez.
Workshop I: “Lentiviral-based gene transduction”
Monday
12-14h Welcome
Introduction to the
courses:
Lentiviral based
gene transduction
14-15h Lunch
15-15:30h Virus
Production I:
* Friday: HEK293T
cells seeding.
Cells Transfection
--------------------------
Tuesday
10-10:30h Virus
Production II
1º Supernatant
collection
10:30-11h Titration
II.
Infection
-MOI
Wednesday
Thursday
Friday
11-11:30 h Virus
Production III.
2º Supernatant
collection.
10-14h Virus
Infection and
Selection in
interesting cells II.
Infection
10-12:30h Virus
Selection in
interesting cells III
Selection and
analysis
11:30-12h Titration
III.
Analysis
-MOI
14-15h Lunch
14-15h Lunch
16-18h Virus
Infection and
Selection in
interesting cells II.
Infection
15-16h Concluding
remarks
14-15h Lunch
12-12:30h Virus
Infection and
Selection in
interesting cells I
Cells Seeding
14-15h Lunch
15:30-16h Titration
I: Cells seeding
Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III
C/ Melchor Fernández Almagro, 3 28029 Madrid
Telf. 00 34 91-4531200 Fax: 00 34 91-4531245 www.cnic.es
Workshop II: “In organello transcription”
Monday
12-14h Welcome
Introduction:
In organello
transcription
Tuesday
11-12h Cells
collection
Wednesday
9h –Gel
preparation
14-15h Lunch
12-14h In organello
transcription
11-16h Gel running
14-15h Lunch
14-15h Lunch
14-15h Lunch
15-15:30h
Cells seeding
14-15h Incubation
16-17:30h
Gel drying
exposition.
15-16h Extraction
Thursday
15-16h
Autoradiography
development
Friday
12:30-14h
Autoradiography
analysis
14-15h Lunch
15-16h Concluding
remarks
and
Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III
C/ Melchor Fernández Almagro, 3 28029 Madrid
Telf. 00 34 91-4531200 Fax: 00 34 91-4531245 www.cnic.es